Related Experiment Video
Updated: Feb 3, 2026
05:35
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
Published on: September 20, 2022
4.2K
Integrated dynamic control and enzyme co-localization strategies enable high-efficiency stilbenoid biosynthesis
Guofu Zhao1, Pengpai Li1, Xinyao Ding1
1MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
Bioresource Technology
|February 1, 2026
Summary
Engineered E. coli efficiently produce stilbenoids like piceatannol and pterostilbene using CRISPRi and enzyme fusion. This microbial biosynthesis offers a sustainable alternative to plant extraction and chemical synthesis for valuable natural products.
Area of Science:
- Metabolic Engineering
- Synthetic Biology
- Natural Product Biosynthesis
Background:
- Stilbenoids are valuable plant-derived polyphenols with significant bioactivities.
- Challenges in stilbenoid production include low natural abundance and toxic chemical synthesis routes.
Purpose of the Study:
- To engineer Escherichia coli for efficient biosynthesis of multiple stilbenoids.
- To overcome limitations in natural product manufacturing through metabolic engineering.
Main Methods:
- Developed a p-coumaric acid-responsive CRISPRi system for dynamic metabolic regulation.
- Employed enzyme co-localization strategies, including dual-enzyme fusion design.
- Utilized orthogonal protein ligation systems (SpyTag/SpyCatcher, SnoopTag/SnoopCatcher) for pathway optimization.
Main Results:
- Achieved highest reported titers for piceatannol (583.10 mg/L) and pterostilbene (1110.92 mg/L) from glycerol with L-tyrosine supplementation.
- Demonstrated significant enhancement of pathway flux through optimized intermediate channeling.
- Techno-economic analysis confirmed the feasibility of microbial stilbenoid manufacturing.
Conclusions:
- The integrated strategy provides a robust platform for microbial stilbenoid biosynthesis.
- This approach offers a sustainable and efficient alternative for producing high-value natural products.
- Presents a valuable paradigm for the biosynthesis of other complex natural products.
Related Concept Videos
02:34Enzymes
94.7K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.7K
01:24Biosynthesis in Bacteria
666
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
666
01:26Biosynthesis of Polysaccharides
640
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
640
01:29Biosynthesis of Lipids
584
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
584
01:28Biosynthesis of Nucleic Acids
1.1K
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
1.1K
01:19Enzyme Kinetics
104.1K
Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
104.1K