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![Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F63188.jpg&w=3840&q=50)
Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy
Published on: April 21, 2023
1H NMR-guided isolation of ophiobolins from Bipolaris maydis and their bioactivities
Yong Shen1, Xiaotian Zhang1, Qin Li1
1Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei Province, People's Republic of China.
Abstract:
Ophiobolins present characteristic 1H NMR resonances, which distinctly differ from other metabolites reported from Bipolaris maydis, and were used for an NMR-guided isolation in this research. As a result, 17 undescribed ophiobolin-sesterterpenoids, ophiobolinoids A-Q (1-17), were isolated from Bipolaris maydis. Ophiobolinoids A-C possessed a rare oxygen bridge connecting C-3 and C-21. Their structures were elucidated by comprehensive spectroscopic analyses, DP4+ calculations, ECD calculations, and X-ray diffraction analyses. Specifically, ophibolinoid C showed a weak inhibitory effect on NO production in LPS-induced RAW264.7 cells.
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