Related Experiment Video
Updated: Feb 3, 2026

Techniques of Sleeve Gastrectomy and Modified Roux-en-Y Gastric Bypass in Mice
Published on: March 20, 2017
Targeted lipidomics reveals distinct mechanisms driving LDL cholesterol response to gastric bypass and sleeve
D Benaiges1, I Lázaro2, A Goday3
1Department of Endocrinology and Nutrition. Hospital del Mar, Passeig Marítim, 25-29, 08003, Barcelona, Spain; Department of Medicine, Universitat Pompeu Fabra, Plaça de la Mercè, 10-12, 08002, Barcelona, Spain; Cardiovascular Risk and Nutrition, Hospital del Mar Research Institute, Dr. Aiguader, 80, 08003, Barcelona, Spain; Consorci Sanitari de l'Alt Penedès i Garraf, Vilafranca del Penedès, Spain; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, Avenida Monforte de Lemos, 3-5. Pabellón 11. Planta 0, 28029, Madrid, Spain.
Background And Aims:
The Bariatric Surgery and LDL Cholesterol (BASALTO) randomized clinical trial reported differential LDL cholesterol responses after Roux-en-Y gastric bypass (RYGB) and sleeve gastrectomy (SG). In this exploratory analysis of BASALTO we characterized lipidomic changes associated with both procedures and their relationship with LDL cholesterol response.
Methods And Results:
Plasma samples of 30 patients with obesity and high LDL cholesterol (15 RYGB, 15 SG) were analyzed using targeted mass spectrometry to quantify over 50 pre-defined lipid species at baseline, 3 months, and 12 months after surgery. At 12 months, RYGB induced consistent reductions in LDL cholesterol, whereas SG showed marked interindividual variability. Both procedures were associated with shared and procedure-specific lipidomic changes, including divergent patterns in cholesterol absorption. No significant associations between lipidomic changes and LDL cholesterol were observed after RYGB. In contrast, after SG, changes in monoacylglycerols (16:0, 20:4, 18:1) at 3 months and increases in cholestanol (pFDR = 0.039) and HexCer 18:0 (pFDR = 0.031) at 12 months were directly associated with LDL cholesterol response.
Conclusion:
LDL cholesterol reduction after RYGB appears independent of shifts in selected lipids, whereas variability after SG may be partly driven by cholesterol absorption and ceramide-related pathways.
Clinical Trial Registration:
NCT03975478.
Related Concept Videos
Target Cell Response to Hormones
Notably, the cellular response can be regulated by altering the number of receptors expressed in the cell. For example, prolonged exposure to elevated hormone levels results in a gradual decline or down-regulation in the number of receptors for that specific hormone on the cell surface. Conversely, in response to low hormone levels, cells may use up-regulation, producing an...
Cholesterol: Significance and Regulation
Considering cholesterol and...
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...
Gastric Motility
Peristaltic Waves and Chyme Formation
Upon food entry, the stomach initiates...
Energy to Drive Translocation
Generally, polypeptides are unfolded by two distinct...
Gastric Emptying

