Semi-Automated and Consistent Fabrication Method of ssDNA-Functionalized Single-Walled Carbon Nanotube Biosensor
Seonghyeon An1, Jaewon Chang1, Yeongjoo Suh2,3
1School of Biomedical Convergence Engineering, Pusan National University, Yangsan, Gyeongsangnam-do, Republic of Korea.
Small Methods
|February 2, 2026
Summary
A new semi-automated platform ensures consistent fabrication of single-walled carbon nanotube (SWCNT) dispersions, crucial for reliable nanosensor development. This automated approach improves batch consistency and reduces manual labor for SWCNT-based nanomaterials.
Area of Science:
- Materials Science
- Nanotechnology
- Biomedical Engineering
Background:
- Single-walled carbon nanotubes (SWCNTs) possess unique optical and electronic properties valuable for various applications.
- Effective dispersion of SWCNTs in liquid media is essential for their use in solution processing and biological systems.
- Traditional manual methods for SWCNT dispersion often lead to inconsistencies due to imprecise weighing and sonication control.
Purpose of the Study:
- To develop a semi-automated platform for consistent fabrication of single-stranded DNA (ssDNA)-wrapped SWCNT dispersions.
- To overcome batch-to-batch variations inherent in manual preparation techniques.
- To enable systematic investigation and optimization of SWCNT dispersion parameters.
Main Methods:
- Integration of an XYZ-motorized stage with a programmable tip sonicator for automated sample processing.
- Precise control over SWCNT mass sourcing and sonication parameters (power, duration).
- Fabrication of ssDNA-wrapped SWCNT dispersions with varying ssDNA:SWCNT mass ratios.
Main Results:
- Achieved unprecedented consistency in SWCNT dispersion fabrication.
- Enabled automated processing of multiple samples, significantly reducing human labor.
- Demonstrated the platform's utility in producing uniform, high-quality serotonin-responsive nanosensors with reliable fluorescence characteristics and sensitivity.
Conclusions:
- Automated fabrication processes are critical for the reliable and scalable production of SWCNT-based nanomaterials.
- The developed platform offers precise control over critical parameters, leading to uniform sensing properties.
- This approach facilitates systematic research into fabrication variables and enhances the performance of SWCNT-based nanosensors.
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