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Updated: Feb 3, 2026
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In Vitro Analysis of E3 Ubiquitin Ligase Function
Published on: May 14, 2021
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Tomato RING Type E3 Ligases, SlRGLGs, Positively Regulate the Dehydration Stress Response.
Yeongil Bae1, Chae Woo Lim1, Dae Sung Kim2
1Department of Life Science (BK21 Program), Chung-Ang University, Seoul, Korea.
Physiologia Plantarum
|February 2, 2026
Summary
Four tomato RING domain ligase (RGLG) proteins positively regulate dehydration stress tolerance. Silencing SlRGLGs increased sensitivity, while overexpression in Arabidopsis enhanced tolerance, highlighting their crucial role in plant water-loss response.
Area of Science:
- Plant Molecular Biology
- Plant Stress Physiology
- Biochemistry
Background:
- The ubiquitin-proteasomal system regulates plant dehydration stress responses.
- RING E3 ligases, including RGLG proteins, are key modulators of this process.
Purpose of the Study:
- To isolate and characterize four tomato SlRGLG proteins (Solanum lycopersicum RING domain ligase).
- To elucidate the molecular and biological functions of SlRGLGs in dehydration stress response.
Main Methods:
- Isolation and sequence analysis of SlRGLG proteins.
- Analysis of transcript levels under dehydration, mannitol, and abscisic acid treatments.
- Subcellular localization of SlRGLGs-GFP fusion proteins.
- In vitro E3 ligase activity assays of MBP-SlRGLGs.
- Generation of SlRGLGs-silenced tomato and SlRGLGs-overexpressing Arabidopsis plants.
Main Results:
- Four SlRGLGs possess conserved VWA and RING domains and show high sequence identity to orthologs.
- SlRGLG transcript levels are responsive to dehydration and related stimuli.
- SlRGLGs exhibit E3 ligase activity and distinct subcellular localization.
- SlRGLG-silenced tomato plants displayed dehydration sensitivity, while SlRGLG-overexpressing Arabidopsis plants showed enhanced tolerance.
Conclusions:
- All four characterized SlRGLGs play a positive role in tomato's dehydration stress response.
- These findings underscore the importance of RGLG proteins in plant adaptation to water-deficit conditions.
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