Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Positive Regulator Molecules

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.

Positive Regulator Molecules

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

Responses to Salt Stress

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

Stress Response System

Stress Response System

The stress response system, also known as the fight-or-flight response, is the body's automatic physiological reaction to perceived threats. Hans Selye introduced the concept of General Adaptation Syndrome (GAS) to describe the predictable pattern of changes that occur in response to stress. GAS consists of three sequential stages: alarm, resistance, and exhaustion. This model helps explain how chronic stress can contribute to health problems.
Alarm stage
In the alarm stage, the body's...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The Triglyceride-Glucose Index and Colorectal Adenoma: A CHungcheong Association for the Study of Intestinal Disease (CHASID) Multi-Center Cross-Sectional Study.

Journal of clinical medicine·2026

Same author

Distinct predictive factors for acute severe ulcerative colitis in East Asian and Western populations.

Inflammatory bowel diseases·2026

Same author

<i>Dokdonella aquatica</i> sp. nov., isolated from freshwater.

International journal of systematic and evolutionary microbiology·2026

Same author

Comparison between ligation-assisted and precutting endoscopic mucosal resection for small rectal neuroendocrine tumors: a CHASID study.

The Korean journal of internal medicine·2026

Same author

Pepper G-type Lectin Receptor-like Kinase, CaRLK1, Modulates ABA-Mediated Stomatal Closure and Drought Tolerance.

The plant pathology journal·2026

Same author

An RGLG E3 Ubiquitin Ligase CaSIRE1 Negatively Regulates ABA-Dependent Drought Tolerance by Destabilizing CaSAP14 in Pepper.

Plant, cell & environment·2026

Search research articles

Related Experiment Video

Updated: Feb 3, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

Tomato RING Type E3 Ligases, SlRGLGs, Positively Regulate the Dehydration Stress Response.

Yeongil Bae¹, Chae Woo Lim¹, Dae Sung Kim²

¹Department of Life Science (BK21 Program), Chung-Ang University, Seoul, Korea.

Physiologia Plantarum

|February 2, 2026

Summary

Four tomato RING domain ligase (RGLG) proteins positively regulate dehydration stress tolerance. Silencing SlRGLGs increased sensitivity, while overexpression in Arabidopsis enhanced tolerance, highlighting their crucial role in plant water-loss response.

Keywords:

E3 ligase RGLG dehydration tomato ubiquitination

More Related Videos

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

Related Experiment Videos

Last Updated: Feb 3, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Functional Characterization of RING-Type E3 Ubiquitin Ligases In Vitro and In Planta

Published on: December 5, 2019

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Evaluation of Substrate Ubiquitylation by E3 Ubiquitin-ligase in Mammalian Cell Lysates

Published on: May 10, 2022

Area of Science:

Plant Molecular Biology
Plant Stress Physiology
Biochemistry

Background:

The ubiquitin-proteasomal system regulates plant dehydration stress responses.
RING E3 ligases, including RGLG proteins, are key modulators of this process.

Purpose of the Study:

To isolate and characterize four tomato SlRGLG proteins (Solanum lycopersicum RING domain ligase).
To elucidate the molecular and biological functions of SlRGLGs in dehydration stress response.

Main Methods:

Isolation and sequence analysis of SlRGLG proteins.
Analysis of transcript levels under dehydration, mannitol, and abscisic acid treatments.
Subcellular localization of SlRGLGs-GFP fusion proteins.
In vitro E3 ligase activity assays of MBP-SlRGLGs.
Generation of SlRGLGs-silenced tomato and SlRGLGs-overexpressing Arabidopsis plants.

Main Results:

Four SlRGLGs possess conserved VWA and RING domains and show high sequence identity to orthologs.
SlRGLG transcript levels are responsive to dehydration and related stimuli.
SlRGLGs exhibit E3 ligase activity and distinct subcellular localization.
SlRGLG-silenced tomato plants displayed dehydration sensitivity, while SlRGLG-overexpressing Arabidopsis plants showed enhanced tolerance.

Conclusions:

All four characterized SlRGLGs play a positive role in tomato's dehydration stress response.
These findings underscore the importance of RGLG proteins in plant adaptation to water-deficit conditions.