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Updated: Feb 3, 2026

In vitro Biofilm Formation in an 8-well Chamber Slide
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In vitro Biofilm Formation in an 8-well Chamber Slide

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In vitro performance of eugenol and cinnamaldehyde on biofilms produced by Candidozyma auris

Débora Silva Marques de Sousa1,2, Gabrielle Pires de Morais Monari3, Cassiana Ferreira da Rosa3

  • 1Department of Chemical and Biological Sciences, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.

Biofouling
|February 2, 2026
PubMed

Insights

Natural compounds eugenol and cinnamaldehyde show promise against the resistant fungus Candida auris. These agents effectively reduced biofilm formation, offering potential new strategies for combating difficult-to-treat infections.

Area of Science:

  • Microbiology
  • Mycology
  • Infectious Diseases

Background:

  • Candida auris exhibits high resistance to antifungals and disinfectants, leading to persistent colonization and high mortality rates.
  • Candida auris forms biofilms, contributing to invasive infections and treatment challenges.
  • Natural products are explored for novel antimicrobial and antifungal activities.

Purpose of the Study:

  • To evaluate the antibiofilm activity of eugenol and cinnamaldehyde against clinical isolates of Candida auris.
  • To assess the impact of these natural compounds on planktonic and biofilm forms of C. auris.

Main Methods:

  • Testing the inhibitory action of eugenol and cinnamaldehyde on planktonic Candida auris.
  • Quantifying the reduction in biofilm biomass after treatment with eugenol and cinnamaldehyde.
  • Measuring the metabolic activity of C. auris isolates during treatment.

Main Results:

  • Both eugenol and cinnamaldehyde demonstrated inhibitory effects on the planktonic form of Candida auris.
  • A significant reduction in biofilm biomass was observed for most clinical isolates.
  • Increased metabolic activity was noted in most isolates during treatment, suggesting potential heteroresistance.

Conclusions:

  • Eugenol and cinnamaldehyde possess antibiofilm activity against clinical isolates of Candida auris.
  • These natural compounds represent potential candidates for developing new therapeutic strategies against C. auris infections.
  • Further research is required to elucidate the observed increase in metabolic activity and its implications for treatment.
Keywords:
C. aurisbiofilmcinnamaldehydeeugenolnatural productsvirulence

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