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Related Concept Videos

Self-Help Support Groups01:28

Self-Help Support Groups

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Self-help support groups are voluntary, community-based organizations that provide a platform for individuals with shared concerns to exchange support, insights, and practical strategies for coping with life challenges. Typically led by group members or paraprofessionals, these groups form a cornerstone of mental health care, especially in reaching populations that are underserved by traditional healthcare systems.
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Related Experiment Video

Updated: Feb 4, 2026

Optimizing the Setup and Conditions for Ex Vivo Electroretinogram to Study Retina Function in Small and Large Eyes
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A mobile system for whole eye perfusion supporting retinal function and surgery.

Maxwell Lohss1,2, Katelin S Samski1, Alkiviades Liasis1

  • 1Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, United States.

Frontiers in Bioengineering and Biotechnology
|February 2, 2026
PubMed
Summary

The Advancing Straight-to-Human Eye Research (ASTHER) system perfuses whole eyes with blood, maintaining retinal function and blood supply ex vivo. This platform shows promise for human tissue research by overcoming sample degradation challenges.

Keywords:
adaptive optics scanning laser ophthalmoscope (AOSLO)electroretinography (ERG)ex-vivo perfused eyefluorescein angiographyfundusisolated perfused eyeliving extracorporeal eyeoptical coherence tomography (OCT)

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Area of Science:

  • Ophthalmology
  • Translational Research
  • Biomedical Engineering

Background:

  • Human donor tissue is valuable for drug development but degrades quickly after death, limiting its use.
  • Maintaining anatomical context and cellular function in post-mortem tissues is a significant challenge in research.

Purpose of the Study:

  • To introduce the Advancing Straight-to-Human Eye Research (ASTHER) system, a novel ex vivo eye perfusion platform.
  • To assess the feasibility of using the ASTHER system to maintain whole eye viability and function using autologous blood.

Main Methods:

  • The ASTHER system was designed for portability and compatibility with surgical techniques.
  • Feasibility trials involved ophthalmic artery cannulation, arterial perfusion, retinal imaging, and electroretinography.
  • The system was tested on Yucatan mini pig eyes and subsequently in a pilot study with donated human eye tissue.

Main Results:

  • The ASTHER platform successfully maintained retinal blood supply and neuronal function in ex vivo mini pig eyes, confirmed by imaging and electroretinography.
  • Retinal surgery was successfully performed on perfused eyes.
  • A pilot study demonstrated the protocol's applicability to donated human eye tissue.

Conclusions:

  • The ASTHER system effectively supports ex vivo eye viability, preserving retinal blood supply and function.
  • This perfusion platform offers a potential solution to the limitations of post-mortem tissue degradation in translational research.
  • Further validation studies are planned to quantify the structural and functional integrity of perfused human eye tissues.