Related Experiment Video
Updated: Feb 4, 2026
Optimizing the Setup and Conditions for Ex Vivo Electroretinogram to Study Retina Function in Small and Large Eyes
Published on: June 27, 2022
A mobile system for whole eye perfusion supporting retinal function and surgery
Maxwell Lohss1,2, Katelin S Samski1, Alkiviades Liasis1
1Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA, United States.
Introduction:
The use of human donor tissue has the potential to accelerate translational research and support the development of effective human medicines. However, post-mortem sample degradation and the loss of anatomical context limits the utility of primary tissue.
Methods:
Here, an ex vivo perfusion platform, the Advancing Straight-to-Human Eye Research (ASTHER) system, was designed to perfuse whole eyes with autologous blood to support ex vivo viability. The system was designed for portability and compatibility with modern surgical techniques.
Results:
Feasibility trials of ophthalmic artery cannulation, arterial perfusion, retinal imaging, and electroretinography with the ASTHER platform were performed.
Discussion:
Qualitative imaging and electroretinography confirmed post-enucleation retinal blood supply and neuronal function in perfused Yucatan mini pig eyes. Retinal surgery was performed in perfused eyes. A pilot study showed the applicability of the minipig enucleation and perfusion protocol in donated human tissue. Future validation studies of the ASTHER platform will quantify perfused tissue structure and function.
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