Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Detergent Purification of Membrane Proteins01:18

Detergent Purification of Membrane Proteins

6.4K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
6.4K
Nucleosome Remodeling02:54

Nucleosome Remodeling

11.2K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.2K
Bone Formation by Intramembranous Ossification01:29

Bone Formation by Intramembranous Ossification

10.7K
Intramembranous ossification is one of the two processes involved in the development of bones within an embryo. The flat bones of the face, most of the cranial bones, and the clavicles are formed via this process. During intramembranous ossification, the bones develop directly from sheets of undifferentiated mesenchymal connective tissue.
The process begins when mesenchymal cells in the embryonic skeleton gather together and differentiate into osteogenic cells, which then develop into ...
10.7K
Bone Remodeling01:40

Bone Remodeling

40.4K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
40.4K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

4.2K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
4.2K
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

81.1K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
81.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Effects of Glucagon-Like Peptide-1 Receptor Agonists and Sodium-Glucose Co-Transporter-2 Inhibitors on Lean Body Mass in Humans: A Systematic Review and Meta-Analysis of Randomised Controlled Trials.

Diabetes/metabolism research and reviews·2026
Same author

The effectiveness of a targeted education intervention to increase protein intake in people with CHD: a pilot feasibility randomised controlled trial.

The British journal of nutrition·2026
Same author

Electronic Actuation of Surface-Immobilized, pH-Responsive DNA Nanoswitches.

ACS applied materials & interfaces·2026
Same author

The Role of Combining Exercise with Pharmacologic Management of Chronic Kidney disease: From Sodium-Glucose Co-Transporter-2 and Glucagon-Like Peptide-1 to Broader Therapeutic Strategies.

Nephron·2026
Same author

Exploring the Barriers Faced by Medical Students and the Impact of Their Involvement in a Widening Participation Charity.

Advances in medical education and practice·2026
Same author

The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states.

Nucleic acids research·2026

Related Experiment Video

Updated: Feb 4, 2026

Detection of Detergent-sensitive Interactions Between Membrane Proteins
10:09

Detection of Detergent-sensitive Interactions Between Membrane Proteins

Published on: March 7, 2018

6.3K

Detergent-Triggered Membrane Remodelling Monitored via Intramembrane Fluorescence Dequenching.

Claudia M F Andrews1, Christopher M Hofmair1, Lauryn Roberts1

  • 1School of Physics, Engineering and Technology, University of York, Heslington, York YO10 5DD, U.K.

ACS Omega
|February 2, 2026
PubMed
Summary

Detergents like Triton X-100 cause significant membrane changes below critical micellar concentration. A new fluorescence assay reveals nanoscale membrane remodeling and toroidal structures preceding solubilization.

More Related Videos

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

8.3K
Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
11:17

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

Published on: February 17, 2014

14.2K

Related Experiment Videos

Last Updated: Feb 4, 2026

Detection of Detergent-sensitive Interactions Between Membrane Proteins
10:09

Detection of Detergent-sensitive Interactions Between Membrane Proteins

Published on: March 7, 2018

6.3K
Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
08:15

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

Published on: July 16, 2018

8.3K
Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes
11:17

Monitoring Changes in Membrane Polarity, Membrane Integrity, and Intracellular Ion Concentrations in Streptococcus pneumoniae Using Fluorescent Dyes

Published on: February 17, 2014

14.2K

Area of Science:

  • Biochemistry
  • Biophysics
  • Membrane Biology

Background:

  • Detergent-induced membrane solubilization is crucial for biotechnology, yet its mechanisms at the nanoscale remain unclear.
  • Existing technologies struggle to assess nanoscopic membrane disruption events, leaving key questions about detergent remodeling and morphological transitions unanswered.

Purpose of the Study:

  • To develop and utilize a sensitive, generalizable assay for probing nanoscale membrane remodeling induced by detergents.
  • To elucidate the mechanistic details and morphological transitions leading to detergent-induced membrane solubilization.

Main Methods:

  • Development of a single-color fluorescence dequenching assay using membrane-integrated fluorophores.
  • Application of fluorescence spectroscopy, time-correlated single photon counting, single vesicle fluorescence lifetime imaging, and scanning electron microscopy.
  • Investigation of Triton X-100's effects on membrane structure at sub-solubilizing concentrations.

Main Results:

  • Triton X-100 induces substantial membrane morphological changes at concentrations below its critical micellar concentration.
  • A transition from spherical vesicles to toroidal structures was observed during the swelling step, preceding solubilization.
  • The fluorescence dequenching assay demonstrated robustness across different probes and conditions, confirming nanoscale sensitivity.

Conclusions:

  • The study refines a multistep model of detergent-induced membrane solubilization.
  • Fluorescence dequenching is established as a valuable tool for detecting conformational intermediates in membrane disruption.
  • The assay platform can interrogate various molecular disruptors impacting membranes, with potential biomedical applications.