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Integration of network toxicology and bioinformatics identifies key targets and pathways in 1,3-butadiene-induced

Zhengbo Huang1, Min Ou2, Guoshun Li3

  • 1School of Nursing, Binzhou Medical University, Yantai, Shandong, China.

International Journal of Environmental Health Research
|February 2, 2026
PubMed

Insights

1,3-Butadiene (1,3-BD) causes kidney damage through unclear molecular pathways. This study identifies key targets like BCL2 and CASP3, revealing their role in both 1,3-BD nephrotoxicity and renal cancer progression.

Area of Science:

  • Environmental Toxicology
  • Bioinformatics
  • Molecular Biology

Background:

  • 1,3-Butadiene (1,3-BD) is a Group 1 carcinogen linked to various health issues.
  • The molecular mechanisms of 1,3-BD-induced nephrotoxicity are not well understood.
  • Understanding these mechanisms is crucial for developing effective interventions.

Purpose of the Study:

  • To elucidate the molecular targets and pathways involved in 1,3-BD-induced nephrotoxicity.
  • To investigate the potential role of identified targets in renal cancer.
  • To provide insights into the dual role of specific genes in toxicity and cancer.

Main Methods:

  • Network toxicology and bioinformatics analyses were employed.
  • Key molecular targets (BCL2, CASP3, MMP9, SIRT1, TNF) were identified.
  • Molecular docking, simulations, CETSA, and TCGA database analysis were utilized for validation and correlation.

Main Results:

  • BCL2, CASP3, MMP9, SIRT1, and TNF were identified as central mediators of renal toxicity.
  • Pathway analyses indicated involvement of oxidative stress, signaling, and apoptosis.
  • These targets demonstrated diagnostic and prognostic significance in renal cancer.

Conclusions:

  • The identified targets (BCL2, CASP3, MMP9, SIRT1, TNF) are crucial in 1,3-BD nephrotoxicity.
  • These genes may also contribute to renal cancer progression, suggesting a shared molecular basis.
  • Findings offer novel insights into 1,3-BD renal injury mechanisms and potential therapeutic targets.
Keywords:
1,3-Butadiene(1,3-BD)bioinformaticsnephrotoxicitynetwork toxicologytargets

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