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Quantitative variations in L chain types in guinea pig antihapten antibodies
In guinea pig purified antihapten antibodies, the proportion of molecules bearing the kappa- or lambda-type of L chains (K or L molecules) may diverge markedly from that found in normal gamma(2)-globulins. This has been evaluated by precipitation of I(131)-labeled antibody preparations using a specific anti-lambda-chain antiserum. Anti-DNP antibodies isolated 3 wk after immunization of guinea pigs with DNP(65)-BGG antibodies, contain less than 1% of L molecules, while in pipsyl antibodies, isolated from the sera of animals immunized with pipsyl-BGG, the proportion of L molecules is significantly greater than in normal gamma(2)-globulins. Anti-DNP antibodies produced against conjugates of this hapten with carriers other than BGG (BSA, OVA, or poly-L-lysine) or with BGG with a small number of DNP groups (DNP(10)-BGG) contained a greater proportion of lambda-chain bearing molecules than anti-DNP antibodies isolated from late sera of guinea pigs immunized with highly conjugated DNP(65)-BGG. An increased percentage of L molecules was detected in preparations of anti-DNP(65)-BGG antibodies isolated early (10 days), when compared to those isolated later during the course of immunization. However, the level of L molecules in all these anti-DNP antibody preparations was always considerably below that present in normal gamma(2)-globulin. The relative amounts of L molecules in distinct immunoglobulin families (gamma(1) and gamma(2)) in antibody preparations isolated from individual animals was remarkably similar.
In guinea pig purified antihapten antibodies, the proportion of molecules bearing the kappa- or lambda-type of L chains (K or L molecules) may diverge markedly from that found in normal gamma(2)-globulins. This has been evaluated by precipitation of I(131)-labeled antibody preparations using a specific anti-lambda-chain antiserum. Anti-DNP antibodies isolated 3 wk after immunization of guinea pigs with DNP(65)-BGG antibodies, contain less than 1% of L molecules, while in pipsyl antibodies, isolated from the sera of animals immunized with pipsyl-BGG, the proportion of L molecules is significantly greater than in normal gamma(2)-globulins. Anti-DNP antibodies produced against conjugates of this hapten with carriers other than BGG (BSA, OVA, or poly-L-lysine) or with BGG with a small number of DNP groups (DNP(10)-BGG) contained a greater proportion of lambda-chain bearing molecules than anti-DNP antibodies isolated from late sera of guinea pigs immunized with highly conjugated DNP(65)-BGG. An increased percentage of L molecules was detected in preparations of anti-DNP(65)-BGG antibodies isolated early (10 days), when compared to those isolated later during the course of immunization. However, the level of L molecules in all these anti-DNP antibody preparations was always considerably below that present in normal gamma(2)-globulin. The relative amounts of L molecules in distinct immunoglobulin families (gamma(1) and gamma(2)) in antibody preparations isolated from individual animals was remarkably similar.