Related Experiment Video
Updated: Feb 4, 2026
08:27
Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.
Published on: November 30, 2022
5.3K
Natural variation in VrERF088 modulates drought tolerance during mungbean germination
Xi Zhang1, Jinyang Liu1, Shicong Li1
1Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
Summary
Drought stress severely impacts mungbean seed germination. A specific mutation in the VrERF088 gene
Area of Science:
- Plant Genetics
- Agricultural Science
- Molecular Biology
Background:
- Drought stress poses a significant threat to global crop production, particularly affecting seed germination and establishment.
- Mungbean (Vigna radiata) is a vital legume crop susceptible to drought during its early growth stages.
Purpose of the Study:
- To identify genetic factors controlling mungbean seed germination under drought stress.
- To develop molecular markers for selecting drought-tolerant mungbean varieties.
Main Methods:
- Whole-genome resequencing of 414 mungbean germplasms to identify single-nucleotide polymorphisms (SNPs).
- Genome-wide association study (GWAS) to locate quantitative trait loci (QTLs) for drought tolerance during germination.
- Development and verification of kompetitive allele-specific PCR (KASP) markers.
- Gene expression analysis and functional validation in *Arabidopsis thaliana*.
Main Results:
- Identified 4,875,142 high-quality SNPs and a significant QTL associated with seed germination under drought stress.
- Developed functional KASP markers for drought tolerance selection.
- Discovered a mutation in the ABA-responsive element (ABRE) of the VrERF088 promoter.
- Demonstrated that VrABI5 activates the Hap1 promoter via the ABRE motif.
- Overexpression of VrERF088 in *Arabidopsis thaliana* conferred hypersensitivity to ABA and reduced drought tolerance.
Conclusions:
- The mutation in the ABRE motif of the VrERF088 promoter is linked to a higher germination rate under drought stress.
- VrERF088 plays a crucial role in ABA signaling and drought response in mungbean.
- The identified genetic resources and markers can aid in breeding drought-resilient mungbean crops.
Related Concept Videos
02:41Responses to Drought and Flooding
12.1K
Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.
12.1K
01:32What is Natural Selection?
129.2K
Natural selection is an evolutionary process in which individuals with survival-promoting traits reproduce at higher rates. These favorable traits become more common within a population or species. Naturally selected traits initially arise via random genetic mutations. In order for selection to occur, there must be variation within a population, the trait controlling the variation must be heritable, and there must be an evolutionary advantage for variation in the trait.
129.2K
01:14What is Variation?
18.5K
Apart from the measures of central tendency, distribution, outliers, and the changing characteristics of data with time, an important characteristic of any data set is its variation or spread. In some data sets, the data values are concentrated closely near the mean; in others, the data values are more widely spread out from the mean.
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
18.5K
01:19Variation
8.0K
An important characteristic of any set of data is the variation in the data. In some data sets, the data values are concentrated closely near the mean; in other data sets, the data values are more widely spread out from the mean. The most common measure of variation, or spread, is the standard deviation, which is the square root of variance.
When independent and dependent variables are plotted on a scatter plot, the slope of a line is a value that describes the rate of change between the two...
When independent and dependent variables are plotted on a scatter plot, the slope of a line is a value that describes the rate of change between the two...
8.0K
02:53Conservative Site-specific Recombination and Phase Variation
6.8K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.8K
01:18Variation of Atmospheric Pressure
4.1K
Change in atmospheric pressure with height is particularly interesting. The decrease in atmospheric pressure with increasing altitude is due to the decreasing gravitational force per unit area as we move away from the surface of the earth.
Assuming the air temperature is constant at a given altitude and that the ideal gas law of thermodynamics describes the atmosphere to a good approximation, one can find the variation of atmospheric pressure with height.
Let p(y) be the atmospheric pressure at...
Assuming the air temperature is constant at a given altitude and that the ideal gas law of thermodynamics describes the atmosphere to a good approximation, one can find the variation of atmospheric pressure with height.
Let p(y) be the atmospheric pressure at...
4.1K