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The virulence regulator Rv3425 mediates stress adaptation and promotes intracellular survival of Mycobacterium

Wei Zhu1, Aikebaier Reheman2, Guanghui Dang3

  • 1National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China.

Archives of Microbiology
|February 2, 2026
PubMed

Insights

The PE/PPE protein Rv3425 enhances Mycobacterium tuberculosis survival under stress and infection. This protein modulates host immune responses, suggesting it as a potential target for tuberculosis diagnostics and therapeutics.

Area of Science:

  • Microbiology
  • Immunology
  • Molecular Biology

Background:

  • Tuberculosis is caused by Mycobacterium tuberculosis, which has a PE/PPE gene family.
  • The function of Rv3425 (PPE57) within this family is largely unknown.

Purpose of the Study:

  • To investigate the role of Rv3425 in Mycobacterium tuberculosis stress adaptation and virulence.
  • To explore Rv3425's impact on host immune responses during infection.

Main Methods:

  • Investigated Rv3425's effect on stress resistance (SDS, pH, H2O2) in vitro.
  • Created a rv3425 knockout strain to assess phenotypic changes and virulence in THP-1 cells and C57BL/6 mice.
  • Performed transcriptional analysis and T-cell recall assays to evaluate immune modulation.

Main Results:

  • Rv3425 enhanced Mycobacterium tuberculosis resistance to SDS, low pH, and hydrogen peroxide.
  • Rv3425 knockout altered colony morphology and attenuated bacterial survival in host cells and mice.
  • Rv3425 suppressed IFN-α and promoted IFN-γ, increasing Mycobacterium tuberculosis antigenicity.

Conclusions:

  • Rv3425 is crucial for Mycobacterium tuberculosis survival and virulence by enhancing stress resistance and modulating host immunity.
  • Rv3425 exhibits immunomodulatory effects, suppressing IFN-α while promoting IFN-γ.
  • Rv3425 represents a potential target for novel tuberculosis diagnostic and therapeutic strategies.
Keywords:
Mycobacterium tuberculosisAntigen presentationIntracellular survivalRv3425Stress resistance

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