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Updated: Feb 4, 2026

Optimizing Sample Preparation for Cryogenic Electron Microscopy
Published on: April 11, 2025
Preparation of Diplonemid Samples for Microscopy
Galina Prokopchuk1,2, Daria Tashyreva3, Orsola Iorillo1,2
1Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic.
Abstract:
This chapter provides a comprehensive set of protocols for preparing diplonemid cells for a range of microscopy applications, including live-cell imaging, fixed-cell fluorescence microscopy, endocytosis assay, immunofluorescence assay (IFA), and fluorescence in situ hybridization (FISH). It presents detailed instructions for slide preparation, cell harvesting, chemical fixation, permeabilization, and fluorescent labelling, with critical steps highlighted throughout. These protocols are designed to be adaptable for different experimental goals, enabling researchers to obtain high-quality imaging data for exploring the cellular biology of diplonemids.
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