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Microbiome Profiling in Chagas Disease: Sample Collection, Sequencing, and Analysis.

Sergio Castañeda1, Juan David Ramírez1,2, Cristina Poveda3,4,5

  • 1Centro de Investigaciones en Microbiología y Biotecnología-UR (CIMBIUR), School of Sciences and Engineering, Universidad del Rosario, Bogotá, Colombia.

Methods in Molecular Biology (Clifton, N.J.)
|February 2, 2026
PubMed
Summary

This study presents a workflow for analyzing the gut microbiome in mice with Chagas disease. Understanding these microbial shifts is crucial for identifying disease progression markers.

Keywords:
Chagas diseaseGut microbiomeHost–microbiota interactionMicrobial diversitySequencingTrypanosoma cruzi

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Area of Science:

  • Microbiology
  • Immunology
  • Parasitology

Background:

  • Chagas disease, caused by Trypanosoma cruzi, results in severe cardiac and gastrointestinal issues.
  • The gut microbiome's role in Chagas disease severity is increasingly recognized, impacting immune and metabolic functions.

Purpose of the Study:

  • To establish a comprehensive workflow for microbiome analysis in Trypanosoma cruzi-infected mice.
  • To enable the identification of microbial signatures linked to Chagas disease progression.

Main Methods:

  • Sample collection from feces and gastrointestinal tissues.
  • DNA extraction, high-throughput sequencing, and rigorous quality control.
  • Bioinformatic analyses including taxonomic profiling, diversity assessment, and network construction.

Main Results:

  • A detailed workflow for microbiome analysis in a Chagas disease mouse model.
  • Methods for exploring microbial functional potential and identifying disease-associated signatures.

Conclusions:

  • The described workflow facilitates in-depth microbiome investigation in Chagas disease.
  • This approach aids in understanding host-microbiome interactions and identifying potential therapeutic targets.