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Updated: Feb 4, 2026
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High-speed Continuous-wave Stimulated Brillouin Scattering Spectrometer for Material Analysis
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Off-Axis Illumination and Epicollection Confocal Brillouin Scattering Microspectroscopy
Hiroharu Yui1, Yuui Fujiyama1, Shu-Hei Urashima1
1Department of Chemistry, Faculty of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku, Tokyo 162-8601, Japan.
Analytical Chemistry
|February 2, 2026
Summary
Confocal Brillouin scattering microspectroscopy (CBS) can now analyze tissue viscoelasticity even through thick barriers. This new optical setup effectively reduces background noise, enabling detailed studies of biological fluids and protein structures.
Area of Science:
- Biophysics
- Optical Spectroscopy
- Materials Science
Background:
- Viscoelastic properties are crucial for biological structure and function.
- Confocal Brillouin scattering microspectroscopy (CBS) offers non-invasive analysis of local viscoelasticity.
- Strong background noise from reflections and scattering limits CBS applications in biological samples.
Purpose of the Study:
- To develop an optical configuration for reducing background noise in CBS.
- To enable quantitative analysis of viscoelastic properties in buried biological samples and fluids.
- To enhance the applicability of CBS in biochemistry and biomedicine.
Main Methods:
- Utilized an off-axis illumination and epicollection optical configuration for CBS.
- Tested the system using aqueous solutions of bovine serum albumin (BSA) with electrolytes.
- Measured samples through thick glass windows (1.25 mm) to simulate challenging biological conditions.
Main Results:
- Successfully reduced background noise from thick glass interfaces.
- Enabled quantitative analysis of Brillouin scattering spectra for BSA solutions up to 20 wt %.
- Identified the contribution of hydrated water molecules to Brillouin scattering bandwidth.
Conclusions:
- The developed optical configuration significantly enhances CBS capabilities for analyzing viscoelastic properties.
- The method is applicable for studying protein conformational changes in fluids.
- This advancement holds promise for assessing complex biological tissues and fluids like blood and lymph.
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