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Updated: Feb 4, 2026

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
Published on: July 10, 2019
A new real-time RT-PCR for the detection of piscine orthoreoviruses PRV-1 and PRV-3
Laurane Pallandre1, Marine Baud1, Emma Rolland1
1Laboratoire de Ploufragan-Plouzané-Niort, ANSES, Plouzané 29280, France.
Abstract:
Piscine orthoreoviruses (PRV) are often associated with pathologies affecting various salmonid species, especially farmed species. Both surveillance and research purposes require diagnostic molecular-based tools able to detect the most frequent genotypes, namely PRV-1 and PRV-3. A new one-step reverse-transcription real-time PCR (RT-qPCR) was set up targeting a conserved region of the L1 segment. Based on the French standard NF U47-600, this PCR method proved to be specific to PRV, excluding other salmonid viruses. The assay can detect 1000 copies of a synthetic transcript of PRV-3, pure or mixed with nucleic acids from healthy fish. This assay produced signals similar to those observed using a previously published generic assay targeting a different viral segment. The new assay is reliable and can be used for the detection of PRV-1 and PRV-3 in rainbow trout and Atlantic salmon.
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