Related Experiment Video For Carbohydrate antigen 199
Updated: Feb 4, 2026
Synthesis and Characterization of Functionalized Metal-organic Frameworks
Published on: September 5, 2014
Highly sensitive chemiluminescence immunoassay utilizing functionalized magnetic metal-organic framework materials
Xiaomin Zuo1, Wenxia Chen2, Xianlong Lu2
1Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, Anhui Province, China.
Background:
In response to the urgent need for highly sensitive and rapid combined detection of multiple tumor markers for the early diagnosis of colorectal cancer, this work innovatively constructs a magnetically-driven chemiluminescence (CL) immunosensing platform (CoFe2O4@ZIF-8/luminol-Au NPs).
Results:
CoFe2O4 NPs were firstly aminated via solvothermal method and lysine modification to provide specific binding sites. ZIF-8 was then grown in situ to form a core-shell structure with high specific surface area for efficient luminol loading. Finally, Au NPs were generated in situ within ZIF-8, simultaneously immobilizing luminol to form composite signal units. This design integrates magnetic separation, confinement effects, and signal amplification, generating an ideal microenvironment for enhanced CL reactions. The label-free CL immunoassay achieves an impressive limit of detection (LOD) of 22 fg/mL for CEA and 5.4 fg/mL for CA199, with broad linear ranges spanning over 5 orders of magnitude. The entire detection process is completed within 30 min, and the analysis of real serum samples demonstrates good recoveries.
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