A chromosome-level genome assembly for the mulberry thrips Pseudodendrothrips mori (Thysanoptera: Thripidae)

The mulberry thrips, Pseudodendrothrips mori Niwa, is a significant agricultural pest whose genomic study has been hindered by a lack of reference data. Here, we present the first high-quality, chromosome-level genome assembly for this pest. The genome was assembled using 10.24 Gb of PacBio HiFi long reads, polished with 16.87 Gb of Illumina short reads, and scaffolded using 17.61 Gb of Hi-C data. The final assembly spans 280.93 Mb, with 98.65% of the sequence anchored into 19 pseudochromosomes. The assembly exhibits exceptional contiguity (contig N50: 1.51 Mb; scaffold N50: 14.52 Mb) and high base-level accuracy (QV: 42.82). We identified 18.32% (51.47 Mb) of the genome as repetitive elements and predicted 13,429 protein-coding genes, of which 98.68% were functionally annotated by NR and Interproscan. The quality of this assembly is further validated by a high BUSCO score (98.0% complete genes from the insecta_odb12 dataset). This genomic resource provides a critical foundation for research into pest genetics, insecticide resistance, and the evolution of Thysanoptera.