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Updated: Feb 4, 2026

Laser Capture Microdissection of Mammalian Tissue
Published on: October 1, 2007
Laser-Capture Microdissection Followed by ATAC-Seq
Celia L Hartmann1,2, Joachim L Schultze2,3,4, Marc D Beyer1,2,3
1Immunogenomics & Neurodegeneration, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) e.V, Bonn, Germany.
Abstract:
Laser-capture microdissection (LCM) enables the collection of regions of interest from target tissues, making it compatible with a number of spatially resolved downstream omics applications. Here, we provide a detailed protocol for LCM coupled to ATAC-seq (LCM-ATAC-seq) for the spatial analysis of chromatin landscape based on the method published by Carraro et al. (Cell Rep Methods 3:100598, 2023). LCM-ATAC-seq allows the efficient capture down to single nuclei and can be combined with the morphological staining of target cell populations to characterize chromatin states in fresh-frozen tissue samples.
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