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Related Experiment Video

Updated: Feb 4, 2026

Laser Capture Microdissection of Mammalian Tissue

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Laser-Capture Microdissection Followed by ATAC-Seq.

Celia L Hartmann^1,2, Joachim L Schultze^2,3,4, Marc D Beyer^1,2,3

¹Immunogenomics & Neurodegeneration, Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE) e.V, Bonn, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|February 2, 2026

Summary

This summary is machine-generated.

This study details a protocol for laser-capture microdissection coupled to ATAC-seq (LCM-ATAC-seq). This method allows spatial analysis of chromatin landscape in fresh-frozen tissues, capturing down to single nuclei.

Keywords:

ATAC-seq Chromatin accessibility Laser-capture microdissection Morphological staining Spatial omics

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Last Updated: Feb 4, 2026

Laser Capture Microdissection of Mammalian Tissue

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Laser Capture Microdissection of Drosophila Peripheral Neurons

Laser Capture Microdissection of Drosophila Peripheral Neurons

Published on: May 24, 2010

ATAC-Seq Optimization for Cancer Epigenetics Research

ATAC-Seq Optimization for Cancer Epigenetics Research

Published on: June 30, 2022

Area of Science:

Molecular Biology
Genomics
Epigenetics

Background:

Laser-capture microdissection (LCM) isolates specific cells from tissue sections.
Spatially resolved omics applications require precise sample collection.
Understanding chromatin accessibility in specific tissue regions is crucial.

Purpose of the Study:

To provide a detailed protocol for LCM coupled to ATAC-seq (LCM-ATAC-seq).
To enable spatial analysis of the chromatin landscape in fresh-frozen tissue samples.
To characterize chromatin states in morphologically defined cell populations.

Main Methods:

Detailed protocol for LCM-ATAC-seq based on Carraro et al. (Cell Rep Methods 3:100598, 2023).
Efficient capture of biological material down to single nuclei.
Integration with morphological staining for cell population characterization.

Main Results:

Successful implementation of LCM-ATAC-seq for spatial epigenomic analysis.
High-efficiency capture of nuclei from fresh-frozen tissues.
Characterization of chromatin states in specific, morphologically identified cell types.

Conclusions:

LCM-ATAC-seq is an effective method for spatial chromatin landscape analysis.
The protocol allows for detailed epigenomic profiling of fresh-frozen tissues.
This technique facilitates the study of cell-type-specific chromatin states in their native context.