Related Experiment Video
Updated: Feb 4, 2026

In vivo Measurement of Knee Extensor Muscle Function in Mice
Published on: March 4, 2021
Can Knee Extensor Loading Deficits Be Diagnosed Using Variables from Force Plates during Squats Post-ACLr?
Jiaqi Wang1, Lori Michener, Kathryn Havens
1Division of Biokinesiology and Physical Therapy, University of Southern California, Los Angeles, CA.
Abstract:
The persistence of knee extensor moment (KEM) deficits across rehabilitation post-ACL reconstruction may be attributed to difficulty detecting and addressing deficits clinically. During squats, large KEM deficits are present along with much smaller differences in joint or segment angles, making them difficult to detect visually. Data outputs from force plates and video technology may improve clinical identification.
Purpose:
Investigate the accuracy of three approaches to estimate KEM deficits using outputs from the force plate and two-dimensional (2D) position data to identify individuals with surgical limb KEM deficits during a bilateral squat.
Methods:
Forty individuals post-ACLr performed bilateral squats. Kinematic and ground reaction force (GRF) data were collected via 3D motion capture system and triaxial force plates. Surgical limb deficits were calculated using limb symmetry index (LSI: nonsurgical/surgical) at peak knee flexion. Gold-standard KEM was calculated using inverse dynamics. Vertical GRF was considered alone, and together with the center of pressure (COP) position to estimate deficits in GRF, and GRF-COP Approaches. Vector Approach used the product of vertical GRF and moment arm calculated using vertical and anterior/posterior GRF, COP, and knee position in trigonometric equations. Separate linear regression and intraclass correlation coefficient (2,k) examined concurrent validity between gold-standard LSI and LSI from GRF, GRF-COP, and Vector Approaches. Specificity and sensitivity (LSI threshold ≥0.85) determined diagnostic accuracy.
Results:
GRF, GRF-COP, and Vector LSI's predicted gold-standard LSI ( R2 = 0.56, 0.74, and 0.85, respectively) with intraclass correlation coefficient (2,k) 0.49, 0.92, and 0.96; sensitivity of 72.2%, 94.4%, 100%, and specificity of 100%, 50%, 100%, respectively.
Conclusions:
Vertical GRF alone is not adequate to detect KEM deficits. Additional force plate and 2D position data (COP position and moment arm) can strengthen predictive ability and diagnostic accuracy, supporting translation of these variables into commercial products to enhance clinical assessments.
Related Concept Videos
Work and Energy for Variable Forces
Fluid Pressure over Flat Plate of Variable Width
The pressure distribution on the plate can be calculated by determining the force that acts on a differential area strip of the plate. Thus, the magnitude of the force is equal to the...
Diagnosing Acidosis and Alkalosis
First, the pH level is assessed to determine whether the blood pH is normal (7.35–7.45), low (acidosis), or high (alkalosis).
Next, the PCO2 and...
Knee Joint
A total of seven ligaments support the knee joint. The patellar ligament, which is also attached to the quadriceps femoris...
Variability: Analysis
The range is a simple measure of variability, indicating the difference between the highest and...
Random Variables
Uppercase letters such as X or Y denote a random variable. Lowercase letters like x or y denote the value of a random variable. If X is a random variable, then X is written in words, and x is given as a number.
For example, let X = the...

