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Updated: Feb 4, 2026

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
Published on: July 7, 2010
Genome-wide analysis of flax bZIP genes using a T2T genome reveals abiotic stress roles
Hang Wang1,2, Jianyu Lu1,2, Shuyao Li1,2
1Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.
Abstract:
The basic leucine zipper (bZIP) transcription factor family plays key roles in plant growth, development, and environmental stress responses but remains poorly characterized in flax (Linum usitatissimum L.). We identified 99 bZIP genes from the telomere-to-telomere (T2T) genome and 92 from the Longya10 reference genome. These genes form 12 conserved subfamilies and contain promoter cis-elements related to phytohormone signaling and abiotic stress responses. Identification of 69 segmentally duplicated gene pairs indicated that large-scale duplication drove bZIP family expansion in flax, while miRNA-target prediction suggested Lus-miR398 as a key regulator of LubZIP transcription factors. Transcriptomic data showed that most bZIP genes were highly expressed in leaves, while RT-qPCR revealed that LubZIP15 and LubZIP45 (subfamilies I and H) were strongly induced by cold, drought, and salt stresses, providing a comprehensive genome-wide view of the flax bZIP family and their potential roles in abiotic stress tolerance.
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