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Updated: Feb 4, 2026

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
Published on: October 12, 2018
Single-Nucleus RNA Sequencing Reveals Cellular Heterogeneity and Trajectories of Lineage Differentiation during
Tao Zhang1,2, Yu Chen1,2, Weilin Chen1,2
1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
None:
Understanding chicken skeletal muscle development is crucial for meat production, yet its cellular and molecular mechanisms are not fully understood. To address this, we built a single-nucleus RNA sequencing atlas of chicken skeletal muscle across key developmental stages (E4-P30) using 111,328 nuclei. We identified 22 cell populations, including nine myogenic ones, revealing extensive heterogeneity and transcriptional diversity. Myonuclei were classified into three fiber-type-associated subpopulations: FGF13+ type IIA fast-twitch, MYH1F+ type IIB fast-twitch, and MYH7B+ type I slow-twitch. Pseudotime analysis reconstructed the developmental trajectory from myogenic progenitors to mature myofibers and highlighted RBM24 as a pivotal regulator. Functional validation confirmed that RBM24 promotes myoblast differentiation while inhibiting proliferation. We further identified putative fiber-type-specific regulators (e.g., TBX15 for IIA, TNNT3 for IIB, and FGFRL1 for I). This study delineates myogenic lineage development and identifies key regulatory genes, offering valuable insights into chicken skeletal muscle biology.
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