Related Experiment Video

Updated: Feb 5, 2026

Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy

Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy

Published on: March 29, 2020

Computed Tomography Radiomic Signatures Associated With Neutrophil Extracellular Trap Enrichment and First-Pass

Briana A Santo^1,2, Tatsat R Patel^1,3, Seyyed M Mousavi Janbeh Sarayi^1,4

¹Canon Stroke and Vascular Research Center University at Buffalo NY.

Journal of the American Heart Association

|February 3, 2026

Abstract

Background:

Radiomic and transcriptomic analyses have independently identified features linked to mechanical thrombectomy (MT) outcomes. Here, we integrated paired radiomics/transcriptomics of stroke clots to identify neutrophil extracellular trap (NET) enrichment as a predictor of first-pass MT success, assessing the potential to noninvasively detect NET enrichment using prethrombectomy computed tomography imaging.

Methods:

We performed radiomic/transcriptomic analyses of 32 stroke clots retrieved by MT. Clots were segmented from pre-MT computed tomography angiography and noncontrast computed tomography scans, and radiomic features (RFs) were extracted using PyRadiomics. Differentially expressed genes were identified between modified first-pass effect (mFPE) success and failure using the criteria of log(fold-change) ≥1.5 and q <0.05. RFs significantly different between mFPE outcomes were identified. A NET enrichment score was computed from expression data, and RFs that differed significantly between low- and high-NET-enriched clots were selected to construct an RF signature predictive of NET enrichment. Immunofluorescence was completed on clots to provide ground truth NET labeling.

Results:

A total of 44 differentially expressed genes were identified between mFPE outcomes. NET formation, neutrophil degranulation, and the NET signaling pathway were among the most enriched gene ontologies in the mFPE failure group, with related genes downregulated in the mFPE success group. Forty RFs were significantly different between mFPE outcomes. Of these, 4 were found to be predictive of clot NET enrichment. Immunofluorescence validated that transcriptomic NET signatures accurately reflected NET presence within clot tissues.

Conclusions:

NET enrichment in clots is associated with reduced mFPE success. With further validation, RFs extracted from prethrombectomy computed tomography imaging may serve as noninvasive biomarkers of clot NET content to aid in preprocedural MT decision-making.

Keywords:

blood immunology ischemic stroke mechanics thrombectomy thrombosis

More Related Videos

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Published on: February 24, 2010

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

A High-throughput Assay to Assess and Quantify Neutrophil Extracellular Trap Formation

Published on: January 29, 2019

Related Concept Videos

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

Imaging Studies III: Computed Tomography

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...

First Pass Effect

First Pass Effect

Presystemic elimination, or the first-pass effect, is the metabolism of drugs that reduces their effective concentration at the site of action. Apart from the first-pass effect, the systemic bioavailability of the drug is also reduced by other factors, including incomplete absorption or chemical degradation of drugs.
Depending on the route of administration, drugs can be metabolized in the liver, intestine, lungs, and vasculature. Orally administered drugs are first absorbed through the...

Social Traps

Social traps are negative situations where people get caught in a direction or relationship that later proves to be unpleasant, with no easy way to back out of or avoid. The concept was orignally introduced by John Platt who applied psychology to Garrett Hardin's "Tragedy of the Commons", where in New England herd owners could let their cattle graze in the common ground. This situation seems like a good idea, but an individual could have an advantage. If they owned...

Predicting Reaction Outcomes

Predicting Reaction Outcomes

Kinetics describes the rate and path by which a reaction occurs. In contrast, thermodynamics deals with state functions and describes the properties, behavior, and components of a system. It is not concerned with the path taken by the process and cannot address the rate at which a reaction occurs. Although it does provide information about what can happen during a reaction process, it does not describe the detailed steps of what appears on an atomic or a molecular level. On the other hand,...

Single-pass Transmembrane Proteins

Single-pass Transmembrane Proteins

Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...

Articles linked to this work by shared authors, journal, and citation graph.

Thunderbolt System for acute ischemic stroke secondary to large vessel occlusion: primary results of the THUNDER study.

Journal of neurointerventional surgery·2026

Implementation and clinical utility of ultra-low-field portable magnetic resonance imaging for postprocedural neurological evaluation in ambulatory neurosurgery: illustrative cases.

Journal of neurosurgery. Case lessons·2026

Role of Perfusion Parameters on Outcomes and Safety of Endovascular Therapy in Posterior Cerebral Artery Stroke.

Stroke·2026

Turnover Time in Neuroangiography Suites: Comparison Between Cases Performed at an Ambulatory Neurosurgery Center Versus a Tertiary Hospital.

Stroke (Hoboken, N.J.)·2026

Thrombectomy in Posterior Circulation Tandem Occlusions: Multicenter Comparative Analysis of Procedural Techniques and Predictors of Clinical Outcomes.

Radiology·2026

End-to-End Autonomous Quantification of Brain Aneurysm and Parent Artery Morphology at CT Angiography.

Radiology. Artificial intelligence·2026

See all related articles