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Anti-Glioma Activity of Artejasminolide Isolated From Artemisia argyi Based on Bioactivity Evaluation and Network
Leyi Fu1, Zhiling Yao1, Jiming Chen1
1School of Pharmacy, Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems and Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou, China.
Abstract:
Seven terpenoids were isolated from Artemisia argyi leaves, including one new triterpenoid featuring a unique terminal diagonal methoxy-substituted side chain and six known analogues. Their structures were confirmed by NMR and HR-ESI-MS spectral analyses, and the absolute stereochemistry of Compound 1 was elucidated via x-ray diffraction analysis. Bioassays revealed that 2 exhibited cytotoxicity against U251 glioma cells (IC50 = 5.6 µM) and potently abrogated cell migration and invasion in a dose-proportional manner by suppressing PI3K/AKT/mTOR phosphorylation, as confirmed by Transwell and Western blot analyses. Bioinformatics analysis further suggested EGFR as a potential upstream target mediating these effects. These findings indicated A. argyi as a possible source of bioactive terpenoids with anti-glioma potential, providing a theoretical basis for further pharmacological studies.
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