A Scoping Review on Mechanically Micronized Adipose-Derived Products: Technological Innovations and Regenerative...

Area of Science:

• Regenerative Medicine
• Biotechnology
• Tissue Engineering

Background:

• Traditional enzymatic digestion of adipose-derived stromal vascular fraction (SVF) presents regulatory and technical challenges.
• SVF is crucial for regenerative medicine applications, including fat grafting.

Purpose of the Study:

• To systematically review and categorize mechanical micronization and purification techniques for adipose-derived SVF.
• To provide plastic surgeons with a guide for selecting mechanical processing methods to optimize clinical outcomes.

Main Methods:

• A scoping review following PRISMA-ScR guidelines.
• Systematic search of PubMed, MEDLINE, and Embase databases.
• Analysis of 89 studies on mechanical processing of adipose tissue.

Main Results:

• Identified three primary mechanical micronization techniques: shuffling-based emulsification, rotating blade systems, and filtration bag systems.
• Summarized four major purification methods: filtration, centrifugation, combined filtration-centrifugation, and fluid-washing.
• Categorized final products into mSVF with adipocytes and mSVF concentrates, each with distinct properties.

Conclusions:

• Mechanical processing is a promising, regulatory-friendly approach for preparing adipose-derived SVF, preserving the adipose niche.
• Optimizing mechanical processing parameters can enhance outcomes in fat grafting and regenerative procedures.
• Further research is needed to tailor mechanical processing for next-generation regenerative products.