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In Vitro Antimycobacterial Activities of Short Peptide-Functionalized Silver Nanoparticle and Its In Silico

Uday Suryakanta1, Sourav Mishra1, Ajit Kumar Dhal1

  • 1School of Biotechnology, KIIT Deemed to be University, Bhubaneswar, Odisha 751024, India.

ACS Applied Bio Materials
|February 3, 2026
PubMed

Insights

Researchers developed peptide-functionalized silver nanoparticles (AgNPs) showing potent antimycobacterial activity against various Mycobacterium strains. These novel AgNPs effectively kill bacteria by disrupting cell walls and DNA, while remaining safe for human cells.

Area of Science:

  • Nanotechnology
  • Microbiology
  • Biochemistry

Background:

  • Antimicrobial resistance poses a significant global health challenge.
  • Efficacy of peptide-functionalized silver nanoparticles (AgNPs) against Mycobacterium species is largely unexplored.
  • Development of novel antimycobacterial agents is crucial.

Purpose of the Study:

  • To synthesize and characterize peptide-functionalized AgNPs as potential antimycobacterial agents.
  • To evaluate the efficacy of these AgNPs against various Mycobacterium strains.
  • To investigate the mechanism of action and safety profile of the developed AgNPs.

Main Methods:

  • One-pot synthesis of AgNPs using peptides as reducing and stabilizing agents.
  • Characterization using HR-TEM, DLS, FTIR, and UV-vis spectroscopy.
  • Antimycobacterial activity testing, minimum inhibitory concentration (MIC) determination, cytotoxicity assays, molecular docking, and molecular dynamics simulations.

Main Results:

  • Positively charged peptide-capped AgNPs demonstrated significant antimycobacterial activity.
  • Linear peptide 2 (LP 2) functionalized AgNP showed the highest efficacy (MIC of 5 μM).
  • AgNP LP 2 induced cell death via membranolysis, oxidative stress, and DNA degradation, inhibited biofilm formation, and showed negligible cytotoxicity to HEK293 cells.

Conclusions:

  • Peptide-functionalized AgNPs are effective antimycobacterial agents.
  • LP 2 functionalized AgNP exhibits potent activity through multiple mechanisms.
  • These novel AgNPs represent promising candidates for future antimycobacterial therapies with a favorable safety profile.
Keywords:
AgNPsFadD32MD simulationsMycobacterium sp.antimycobacterialmolecular dockingpeptides

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