Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Metabolism?00:52

What is Metabolism?

131.9K
Overview
131.9K
Carbohydrate Metabolism01:36

Carbohydrate Metabolism

14.2K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
14.2K
What is Evolutionary History?02:35

What is Evolutionary History?

43.6K
Scientists record evolutionary history by analyzing fossil, morphological, and genetic data. The fossil record documents the history of life on Earth and provides evidence for evolution. However, both fossil and living organisms offer evidence that outlines Earth’s evolutionary history.
43.6K
Overview of Metabolism01:40

Overview of Metabolism

38.4K
Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...
38.4K
Regulation of Metabolism01:19

Regulation of Metabolism

11.6K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
11.6K
Metabolic Rate01:25

Metabolic Rate

1.2K
The human body is a powerhouse of energy, with every cell performing numerous functions that require energy. This energy production and consumption is measured by the metabolic rate, which quantifies the total heat generated by all the body's chemical reactions and mechanical work. This measurement helps to determine the rate of kilocalorie (kcal) consumption needed to fuel all ongoing activities.
The Basal Metabolic Rate (BMR) measures the energy expended at rest.
Several factors influence...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The association between preoperative pseudocapsule integrity on magnetic resonance imaging and the pathological features of clear cell renal cell carcinoma.

Abdominal radiology (New York)·2026
Same author

Sex-specific effects of depression on the incidence and risk factors for coronary heart disease: insights from multi-omics approaches.

European journal of preventive cardiology·2026
Same author

Development and external validation of a dual-stage predictive model for self-reported physician-diagnosed chronic lung disease, asthma, and lung cancer in Chinese adults.

Scientific reports·2026
Same author

A Biomimetic Copper-Caffeic Acid Nanozyme Activates Cuproptosis and Pyroptosis by Mimicking the Neutrophil Enzymatic Cascade.

Advanced healthcare materials·2026
Same author

Imipramine inhibits feline infectious peritonitis virus infection by targeting host cholesterol metabolism via NPC1.

Antimicrobial agents and chemotherapy·2026
Same author

Model-Informed Speech Enhancement Using Virtual Room Acoustics and Acoustic Descriptor Optimization.

Sensors (Basel, Switzerland)·2026

Related Experiment Video

Updated: Feb 5, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism
07:36

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

5.3K

A Novel Metabolic Pathway Design Method Based on Evolutionary Algorithms and Metabolic Network Evaluation.

Xin Zhao1, Xueying Sun1, Tao Zhang1

  • 1School of Electrical and Information Engineering, Tianjin University, Tianjin 300072, China.

Journal of Chemical Information and Modeling
|February 3, 2026
PubMed
Summary
This summary is machine-generated.

Metabolic engineers can now improve microbial synthesis with EA-MNE (Evolutionary Algorithm-based Metabolic Network Evaluation). This method accounts for branching reactions, enhancing pathway design accuracy and microbial system efficiency.

More Related Videos

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

25.0K
Metabolic Profile Analysis of Zebrafish Embryos
05:41

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

20.5K

Related Experiment Videos

Last Updated: Feb 5, 2026

Autofluorescence Imaging to Evaluate Cellular Metabolism
07:36

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

5.3K
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

25.0K
Metabolic Profile Analysis of Zebrafish Embryos
05:41

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

20.5K

Area of Science:

  • Metabolic Engineering and Synthetic Biology
  • Computational Biology and Bioinformatics

Background:

  • Metabolic pathway design is key for microbial synthesis of valuable compounds.
  • Current tools often overlook branching reactions, leading to suboptimal pathway selection.
  • Branching reactions divert metabolic flux, reducing target compound yield.

Purpose of the Study:

  • To introduce a novel metabolic pathway design method, EA-MNE, that addresses branching reactions.
  • To improve the accuracy of pathway evaluation and synthetic efficiency in microbial systems.
  • To develop new criteria for assessing the impact of branching reactions.

Main Methods:

  • Developed EA-MNE (Evolutionary Algorithm-based Metabolic Network Evaluation).
  • Proposed a method to expand linear pathways into metabolic networks.
  • Introduced two new evaluation criteria: effective branching reactions and network theoretical yield.
  • Integrated four criteria: branching reactions, network theoretical yield, network toxicity, and Gibbs free energy.

Main Results:

  • EA-MNE systematically addresses challenges posed by branching reactions.
  • The method enhances the accuracy of metabolic pathway evaluation.
  • Improved synthetic efficiency in microbial systems is achieved.
  • New criteria quantify yield losses and branching impacts effectively.

Conclusions:

  • EA-MNE offers a systematic solution for metabolic pathway design considering branching reactions.
  • The method significantly improves the accuracy of pathway evaluation.
  • This leads to enhanced synthetic efficiency in microbial production systems.