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Calcium Dobesilate Interference in Two Urinary Total Protein Detection Methods
Zheng Mingqi1,2, Ma Xiaoli1, Yu Songlin1
1Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
Background:
This study aimed to determine the potential interference of calcium dobesilate in two urinary total protein detection methods.
Methods:
Urine samples from patients receiving calcium dobesilate were collected, and drug concentrations were quantified using liquid chromatography-tandem mass spectrometry. Paired-difference testing was used to assess if calcium dobesilate interfered with the total protein concentration measurement. The interference effect of calcium dobesilate on the detection method was evaluated using dose-effect experiments.
Results:
In total, 22 24-h urine samples and 50 spot urine samples were collected. The calcium dobesilate concentrations ranged from 76 to 738 mg/L (median: 243 [161, 328] mg/L) and 60-2236 mg/L (median: 370 [195, 667] mg/L) in the 24-h and spot urine samples, respectively. Paired-difference testing showed that the total protein results deviated by 0.8%-12.1% and 13.0%-286.4% with the pyrogallol red-molybdate and benzethonium chloride methods, respectively, when the calcium dobesilate concentration was 4500 mg/L. The dose-response experiment with the benzethonium chloride method demonstrated a "J-shaped" effect; negative interference on the total protein concentration occurred at low and medium calcium dobesilate concentrations, but positive interference occurred at high calcium dobesilate concentrations.
Conclusions:
Calcium dobesilate did not significantly interfere with the pyrogallol red-molybdate method but did significantly interfere with the benzethonium chloride method. The direction and degree of interference were related to the urinary protein and drug concentrations. Discontinuing calcium dobesilate for 1-2 days before obtaining a urine sample for total protein detection is recommended.
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