Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Interference01:23

RNA Interference

28.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.1K
Interference and Diffraction02:18

Interference and Diffraction

52.5K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
52.5K
Interference and Decay01:16

Interference and Decay

476
Forgetting is a complex cognitive phenomenon influenced by several factors, among which interference and decay are particularly prominent. These processes explain why individuals often struggle to retrieve specific information from memory, leading to lapses in recall that can be observed in everyday situations.
Interference occurs when competing memories hinder the retrieval of particular information. It can be classified into two types: proactive and retroactive interference. Proactive...
476
Introduction to Urinary System01:13

Introduction to Urinary System

9.1K
The urinary system consists of two kidneys, two ureters, the urinary bladder, and the urethra.
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...
9.1K
Urinary Bladder01:23

Urinary Bladder

3.5K
The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...
3.5K
Disorders of the Urinary System01:20

Disorders of the Urinary System

1.2K
The urinary system is responsible for eliminating waste and excess fluids from the body. However, disorders of the urinary system can arise due to various reasons like infections, stress, age, congenital abnormalities, and lifestyle.
Urinary tract infections (UTIs) are one of the most common urinary system disorders. They are caused by bacteria that enter the urethra and can spread to the bladder resulting in cystitis. Pyelonephritis is the result of a UTI that has ascended to the level of the...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Designing and Evaluating an Autoverification RCV-Based System for Thyroid Function Profiles.

Diagnostics (Basel, Switzerland)·2026
Same author

PAFAH1B3 Exists in Linear Chromosomal and Extrachromosomal Circular DNA and Promotes HCC Progression via EMT.

International journal of molecular sciences·2025
Same author

Decoding crosstalk between neurotransmitters and α-synuclein in Parkinson's disease: pathogenesis and therapeutic implications.

Therapeutic advances in neurological disorders·2025
Same author

Reasons and Resolutions for Inconsistent Variant Interpretation.

Human mutation·2025
Same author

Development and Validation of a Rapid Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantitation of Vitamin K Metabolites in Different Matrices.

Journal of mass spectrometry : JMS·2025
Same author

Molecular Signatures of Cancer Stemness Characterize the Correlations with Prognosis and Immune Landscape and Predict Risk Stratification in Pheochromocytomas and Paragangliomas.

Bioengineering (Basel, Switzerland)·2025
Same journal

Changes of CEA Diagnostic Performance Under the New Reference Interval Stratified by Age and Sex.

Journal of clinical laboratory analysis·2026
Same journal

Investigation of Specific IgG-Secreting Cells in Congenital Toxoplasmosis: The TOXODIAG Study.

Journal of clinical laboratory analysis·2026
Same journal

Development and Analytical Validation of a Laboratory-Customized TaqMan-MGB Probe Method for CYP2C19 Genotyping.

Journal of clinical laboratory analysis·2026
Same journal

Heavy Metal Mixture Exposure and Insulin Resistance in U.S. Adults: The Mediating Role of Systemic Inflammation.

Journal of clinical laboratory analysis·2026
Same journal

A New Diagnostic Approach Using the NLR/LMR Ratio in the Differential Diagnosis of Neck Masses: Hematologic Evaluation of Patients With Cervical Lymphadenopathy.

Journal of clinical laboratory analysis·2026
Same journal

Elevated Hemoglobin A<sub>2</sub>: A Molecular Revisited, and Implications to β-Thalassemia Screening.

Journal of clinical laboratory analysis·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

A Modified Precipitation Method to Isolate Urinary Exosomes
05:05

A Modified Precipitation Method to Isolate Urinary Exosomes

Published on: January 16, 2015

18.0K

Calcium Dobesilate Interference in Two Urinary Total Protein Detection Methods.

Zheng Mingqi1,2, Ma Xiaoli1, Yu Songlin1

  • 1Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.

Journal of Clinical Laboratory Analysis
|February 4, 2026
PubMed
Summary
This summary is machine-generated.

Calcium dobesilate does not interfere with the pyrogallol red-molybdate method for urinary total protein detection. However, it significantly interferes with the benzethonium chloride method, affecting results based on drug and protein concentrations.

Keywords:
benzethonium chloridecalcium dobesilateinterferencetotal urine proteinurine samples

More Related Videos

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis
07:45

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Published on: February 9, 2021

4.0K
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
08:31

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Published on: October 15, 2013

30.4K

Related Experiment Videos

Last Updated: Feb 5, 2026

A Modified Precipitation Method to Isolate Urinary Exosomes
05:05

A Modified Precipitation Method to Isolate Urinary Exosomes

Published on: January 16, 2015

18.0K
Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis
07:45

Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis

Published on: February 9, 2021

4.0K
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
08:31

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

Published on: October 15, 2013

30.4K

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Pharmacology

Background:

  • Urinary total protein measurement is crucial for diagnosing and monitoring kidney diseases.
  • Calcium dobesilate is a medication that may be present in urine samples.
  • Potential interference of medications in diagnostic assays needs careful evaluation.

Purpose of the Study:

  • To investigate the interference of calcium dobesilate in two common urinary total protein detection methods.
  • To quantify the extent and nature of interference caused by varying calcium dobesilate concentrations.

Main Methods:

  • Collected 24-h and spot urine samples from patients on calcium dobesilate.
  • Quantified drug concentrations using liquid chromatography-tandem mass spectrometry.
  • Assessed interference using paired-difference testing and dose-effect experiments.

Main Results:

  • Calcium dobesilate did not significantly interfere with the pyrogallol red-molybdate method.
  • Significant interference was observed with the benzethonium chloride method, showing a "J-shaped" effect.
  • Interference varied with calcium dobesilate and urinary protein concentrations, ranging from -12.1% to +286.4% deviation.

Conclusions:

  • The pyrogallol red-molybdate method is reliable for urinary total protein detection in patients taking calcium dobesilate.
  • The benzethonium chloride method is susceptible to interference from calcium dobesilate, impacting accuracy.
  • Discontinuing calcium dobesilate for 1-2 days prior to urine collection is recommended when using the benzethonium chloride method.