Related Experiment Video
Updated: Feb 5, 2026

Establishment of a Co-culture System of Patient-Derived Colorectal Tumor Organoids and Tumor-Infiltrating Lymphocytes (TILs)
Published on: June 27, 2025
Arginase 2 Promotes Colorectal Cancer Metastasis via PI3K/AKT Pathway Activation and Regulates Tumor Immune
Yueyan Zhang1,2, Ning Qu1,2, Jiale Mei1,2
1Colorectal and Anal Disease Unit, Department of Gastrointestinal Surgery, Guangxi Medical University Cancer Hospital, Nanning, Guangxi, China.
Background:
Colorectal cancer (CRC) is a leading cause of cancer-related deaths, with metabolic reprogramming involved in its pathogenesis. Aberrant Arginase 2 (ARG2) expression is linked to malignant progression, but its role in CRC remains unclear.
Methods:
ARG2 expression in CRC and adjacent tissues was analyzed. In vitro experiments were performed after ARG2 knockdown. Mechanistic investigations focused on epithelial-mesenchymal transition (EMT), PI3K/AKT pathway, immune infiltration, and drug sensitivity.
Results:
ARG2 was upregulated in CRC tissues, correlating with poor prognosis. ARG2 knockdown inhibited CRC cell proliferation, migration, and invasion by reducing Zinc Finger E-Box Binding Homeobox 1 (ZEB1), N-cadherin, and MMP2, suppressing EMT. Additionally, ARG2 knockdown significantly inhibited the PI3K/AKT signaling pathway. Immune infiltration analysis revealed high ARG2 expression correlated with reduced activated B cells and macrophages. Drug sensitivity analysis indicated that high ARG2 expression was associated with decreased efficacy of certain chemotherapeutic agents.
Conclusion:
ARG2 is an independent prognostic marker for CRC. It promotes CRC progression via regulating EMT and PI3K/AKT, holding potential as a novel diagnostic and therapeutic target.
More Related Videos
07:15Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
Published on: August 23, 2024
07:59Portal Vein Injection of Colorectal Cancer Organoids to Study the Liver Metastasis Stroma
Published on: September 3, 2021
Related Concept Videos
PI3K/mTOR/AKT Signaling Pathway
Active versus Passive Immunity
Active Immunity
Active immunity refers to the resistance one develops...
The Eukaryotic Promoter Region
GTPases and their Regulation
Large G-proteins,...
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...
Master Transcription Regulators