Related Experiment Video
Updated: Feb 5, 2026
08:56
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
Published on: December 5, 2016
11.3K
RBM15 Enhances 5-Fluorouracil Drug Sensitivity and Suppresses Gastric Cancer Progression by Modulating
Xingyu Zhu1,2,3, Hao Chen4,5, Kang Xu6
1Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250021, China.
Research (Washington, D.C.)
|February 4, 2026
Summary
High RBM15 expression predicts better gastric cancer prognosis and suppresses tumor growth. RBM15 enhances chemosensitivity by regulating the IGF2BP3-ECT2 axis via m6A methylation.
Area of Science:
- Oncology
- Molecular Biology
- Epigenetics
Background:
- Gastric cancer (GC) is a major global health concern with high mortality rates.
- Identifying prognostic biomarkers and therapeutic targets is crucial for improving GC outcomes.
Purpose of the Study:
- To investigate the role of RNA-binding motif protein 15 (RBM15) in gastric cancer.
- To elucidate the molecular mechanisms underlying RBM15's function and its impact on chemosensitivity.
Main Methods:
- Analysis of clinical tissues and public datasets (TCGA, ACRG, etc.).
- In vitro and in vivo functional assays.
- RNA sequencing, bioinformatics analysis, MeRIP, RIP-qPCR, RNA pull-down, and luciferase reporter assays.
- Animal and patient-derived organoid models.
Main Results:
- High RBM15 expression correlates with favorable prognosis in GC patients.
- RBM15 suppresses GC cell proliferation, migration, and invasion.
- RBM15 targets the oncogene ECT2 via m6A methylation, modulating its interaction with IGF2BP3.
- RBM15 enhances GC sensitivity to 5-fluorouracil (5-FU) chemotherapy.
Conclusions:
- A novel RBM15/IGF2BP3-ECT2 signaling axis regulates epithelial-mesenchymal transition (EMT) and chemosensitivity in GC.
- This axis functions through m6A methylation of ECT2 mRNA.
- RBM15 represents a potential therapeutic strategy for enhancing GC chemosensitivity.
Related Concept Videos
01:17Drug Dependence
1.7K
Medications are typically administered to achieve therapeutic effects. Some drugs can modify an individual's mood and perception, frequently resulting in various enjoyable experiences. However, this can result in drug dependency, a condition marked by continuous drug use despite potential negative consequences. Drug dependency primarily falls into two categories: psychological and physical dependence. Psychological dependence occurs when the pleasurable feelings induced by the drug...
1.7K
03:03mTOR Signaling and Cancer Progression
4.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.8K
01:16Bioavailability Enhancement: Drug Solubility Enhancement
261
Body:Bioavailability is a critical factor in determining a drug's effectiveness. It refers to the proportion of a drug that enters the circulation when introduced into the body and is, as a result, able to have an active effect. Enhancing bioavailability is essential for drugs with poor solubility, as it can significantly impact their therapeutic efficacy. Various methods are employed to increase the solubility of drugs, thereby enhancing their bioavailability.Micronization and nanonization are...
261
01:27Bioavailability Enhancement: Drug Permeability Enhancement
205
Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...
205
01:05Bioavailability Enhancement: Drug Stability Enhancement and GI Retention
219
Body:Improving a drug's stability in the gastrointestinal (GI) tract is paramount for enhancing its bioavailability and therapeutic effectiveness. Various strategies are employed to protect the drug from the harsh gastric milieu and to ensure its release and absorption at the desired site within the GI tract.Polymer coatings are one such method used to shield drugs from the stomach's acidic environment. By preventing premature drug release, these coatings improve the bioavailability of unstable...
219
01:31Acid Suppressive Drugs for Peptic Ulcer Disease: Antacids
704
In the complex environment of the gastric lumen, excessive acid secretion can lead to the formation or worsening of ulcers within the delicate mucosal layer. Antacids, such as sodium bicarbonate and calcium carbonate, provide relief by neutralizing this acid, transforming it into harmless salt and water. This neutralization process raises the gastric pH from a highly acidic level of 1 to a more basic 3-4, reducing the acidity within the stomach.
However, this neutralization reaction between...
However, this neutralization reaction between...
704