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Updated: Feb 5, 2026

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
Published on: September 12, 2019
Atomic force microscopy-based photothermal infrared microscopy for aqueous environments using graphene-based
Yasuhiko Fujita1, Mariko Takahashi1, Hirohmi Watanabe1
1Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST) Kagamiyama 3-11-32 Higashihiroshima 739-0046 Japan Yasuhiko.fujita@aist.go.jp.
Abstract:
We present the first demonstration of atomic force microscopy-based photothermal-induced resonance (PTIR) measurements of hydrated polymers under aqueous conditions, utilizing microfluidic cells with a graphene layer as an atomically thin IR-transparent window. Our findings show that polymer swelling can be successfully detected through changes in the PTIR spectrum.
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