Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.5K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.5K
Leveling Effect and Non-Aqueous Acid-Base Solutions02:11

Leveling Effect and Non-Aqueous Acid-Base Solutions

9.5K
This lesson defines the leveling effect in acidic and basic solutions and its role in aqueous and non-aqueous solutions. It is essential to understand the competing nature of various species in a chemical system.
The Leveling Effect of a Solvent
A generic acid (HA) reacts with the generic base (B-) to yield the corresponding conjugate base (A-) and conjugate acid (HB):
9.5K
Chemical Reactions in Aqueous Solutions03:03

Chemical Reactions in Aqueous Solutions

72.5K
Chemical substances interact in many different ways. Certain chemical reactions exhibit common patterns of reactivity. Due to the vast number of chemical reactions, it becomes necessary to classify them based on the observed patterns of interaction.
72.5K
Transmission-based Precautions II: Airborne and Protective Environment01:25

Transmission-based Precautions II: Airborne and Protective Environment

2.0K
Transmission-based precautions are for patients infected or suspected to be infected (or colonized) with organisms posing a significant risk to others. The transmission precautions include airborne and protective environment precautions.
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
2.0K
Acids, Bases and Neutralization Reactions03:26

Acids, Bases and Neutralization Reactions

63.8K
An acid-base reaction is one in which a hydrogen ion, H+, is transferred from one chemical species to another. Such reactions are of central importance to numerous natural and technological processes, ranging from the chemical transformations within cells or lakes and oceans to the industrial-scale production of fertilizers, pharmaceuticals, and other substances essential to the society.
63.8K
Atomic Orbitals02:44

Atomic Orbitals

44.0K
An atomic orbital represents the three-dimensional regions in an atom where an electron has the highest probability to reside. The radial distribution function indicates the total probability of finding an electron within the thin shell at a distance r from the nucleus. The atomic orbitals have distinct shapes which are determined by l, the angular momentum quantum number. The orbitals are often drawn with a boundary surface, enclosing densest regions of the cloud.
44.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Divergent self-assembly dynamics of squalene-derived bolaamphiphiles enabled by ethylene glycol-length-switchable Ostwald ripening.

RSC advances·2026
Same author

Drugging the redox-regulated immunoproteome.

Nature chemical biology·2026
Same author

TEM analysis of dicarboxylic acid-induced transition from unilamellar to multilamellar MEL-A vesicles.

Nanoscale horizons·2026
Same author

Intermetallic nanoassemblies potentiate systemic STING activation.

Science (New York, N.Y.)·2026
Same author

Nanowire-based AFM-IR microscopy: Unveiling chemical structure at sub-10-nm resolution with silver nanowire-functionalized AFM probes.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Correction to "Biocompatible Label-Free Detection of Carbon Black Particles by Femtosecond Pulsed Laser Spectroscopy".

Nano letters·2026

Related Experiment Video

Updated: Feb 5, 2026

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

10.3K

Atomic force microscopy-based photothermal infrared microscopy for aqueous environments using graphene-based

Yasuhiko Fujita1, Mariko Takahashi1, Hirohmi Watanabe1

  • 1Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST) Kagamiyama 3-11-32 Higashihiroshima 739-0046 Japan Yasuhiko.fujita@aist.go.jp.

Nanoscale Advances
|February 4, 2026
PubMed
Summary

We demonstrate atomic force microscopy-based photothermal-induced resonance (PTIR) for hydrated polymers in water. This technique detects polymer swelling by analyzing changes in the PTIR spectrum, offering new insights into polymer behavior.

More Related Videos

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

1.2K
Bacterial Immobilization for Imaging by Atomic Force Microscopy
10:03

Bacterial Immobilization for Imaging by Atomic Force Microscopy

Published on: August 10, 2011

17.8K

Related Experiment Videos

Last Updated: Feb 5, 2026

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

10.3K
High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping
08:59

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Published on: March 22, 2024

1.2K
Bacterial Immobilization for Imaging by Atomic Force Microscopy
10:03

Bacterial Immobilization for Imaging by Atomic Force Microscopy

Published on: August 10, 2011

17.8K

Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Spectroscopy

Background:

  • Studying hydrated polymers in aqueous environments presents challenges for traditional characterization techniques.
  • Atomic force microscopy (AFM) offers high-resolution surface imaging, but integrating it with spectroscopic methods in liquid is complex.

Purpose of the Study:

  • To develop and demonstrate a novel method for analyzing hydrated polymers in water using AFM-based photothermal-induced resonance (PTIR).
  • To investigate the potential of PTIR to detect changes in polymer structure, such as swelling, under aqueous conditions.

Main Methods:

  • Utilized microfluidic cells with an atomically thin graphene layer as an IR-transparent window.
  • Performed photothermal-induced resonance (PTIR) measurements on hydrated polymers within the microfluidic cells.
  • Analyzed spectral changes to identify polymer swelling.

Main Results:

  • Successfully demonstrated PTIR measurements on hydrated polymers in an aqueous environment.
  • Observed distinct changes in the PTIR spectrum correlated with polymer swelling.
  • Validated the use of graphene as an effective IR-transparent window for microfluidic PTIR.

Conclusions:

  • AFM-based PTIR is a viable technique for characterizing hydrated polymers in aqueous solutions.
  • PTIR can effectively detect polymer swelling by analyzing spectral shifts.
  • This method opens new avenues for studying polymer dynamics and interactions in biologically relevant conditions.