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E. C. Tolman emphasized the purposiveness of behavior — the idea that much of our behavior is goal-directed. For instance, employees who aim for a promotion work diligently to meet their targets. Tolman argued that when classical conditioning and operant conditioning occur, the organism acquires certain expectations. In classical conditioning, a child might fear a dog because they expect it to bite. In operant conditioning, a person might consistently work overtime because they expect a...
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Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos
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Mycobacterium abscessus research: learning from challenges.

Ryan Z Treen1, Mercedes Gonzalez-Juarrero2, Mary Jackson2

  • 1Wadsworth Center, New York State Department of Health, Albany, New York, USA.

Journal of Bacteriology
|February 4, 2026
PubMed
Summary
This summary is machine-generated.

Mycobacterium abscessus (Mab) infections are rising, with significant genetic variations between strains. New research frameworks are needed for accurate in silico, in vitro, and animal model studies of this antibiotic-resistant pathogen.

Keywords:
Mycobacterium abscessusNon-tuberculous mycobacteria

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Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Mycobacterium abscessus (Mab) is a rapidly growing mycobacterium causing difficult-to-treat infections.
  • Clinical cases and research publications on Mab have significantly increased.
  • The reference strain ATCC19977 is widely used but may not represent clinical isolates due to genetic variability.

Purpose of the Study:

  • To highlight challenges in Mycobacterium abscessus research due to genetic diversity.
  • To propose a framework for future Mab studies.
  • To introduce a new web-based resource for pangenome analysis.

Main Methods:

  • Whole-genome sequence analyses.
  • Transposon-seq based functional genomics.
  • Pangenome analysis using a new web-based resource.

Main Results:

  • Mycobacterium abscessus exhibits an open pangenome with substantial genetic variations.
  • Essential genes differ between the reference strain ATCC19977 and clinical isolates.
  • These variations necessitate careful strain and condition selection in experimental designs.

Conclusions:

  • Future Mycobacterium abscessus research requires acknowledging and addressing significant genetic diversity.
  • A proposed framework guides in silico, in vitro, and animal model studies.
  • Standardized approaches and resources like the new pangenome analysis tool are crucial for advancing Mab research.