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Updated: Feb 6, 2026
08:23
Author Spotlight: Optimization of Performance Parameters of the TAGGG Telomere Length Assay
Published on: April 21, 2023
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TARPON-A Telomere Analysis and Research Pipeline Optimized for Nanopore
Nathaniel Deimler1,2, David V Ho1,2, Norbert Paul3
1Department of Biology, Johannes Gutenberg University, Mainz, Germany.
Plos Computational Biology
|February 4, 2026
Summary
TARPON is a new pipeline for analyzing telomeres using Oxford Nanopore sequencing. This tool simplifies telomere length analysis for researchers, even those without extensive bioinformatics experience.
Area of Science:
- Genomics
- Molecular Biology
Background:
- Long-read sequencing offers nucleotide-level telomere length analysis.
- Clinical adoption requires validation of new sequencing technologies.
Purpose of the Study:
- Introduce TARPON, a Nextflow pipeline for Nanopore-based telomere analysis.
- Provide a validated, user-friendly tool for telomere research.
Main Methods:
- Developed a Nextflow pipeline (TARPON) for Oxford Nanopore Technologies (ONT) data.
- Implemented read filtering, strand specificity assignment, and quality control.
- Enabled customizable statistics and visualizations.
Main Results:
- TARPON isolates telomeric reads and defines telomere boundaries.
- The pipeline excludes low-quality and non-telomeric reads.
- Generated publication-ready outputs for diverse applications.
Conclusions:
- TARPON is the first complete, validated pipeline for Nanopore telomere analysis.
- Offers ease of use for beginners and flexibility for advanced users.
- Supports telomere analysis in various samples and organisms.
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