Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Cellulose and Pectic Polysaccharides

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...

Optimal Foraging

Optimal Foraging

How animals obtain and eat their food is called foraging behavior. Foraging can include searching for plants and hunting for prey and depends on the species and environment.

Optimization Problems

Optimization Problems

Optimization problems often involve identifying maximum or minimum values under specific constraints. A well-known example is determining the longest horizontal pipe that can be moved around a right-angled corner, where a 3-meter-wide hallway meets a 2-meter-wide hallway. This scenario, common in architectural design and industrial transport, can be understood conceptually through geometric and trigonometric reasoning.To visualize the problem, consider the pipe as a straight line that touches...

Structures of Solids

Structures of Solids

Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...

Structural Isomerism

Structural Isomerism

Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chemical constituents of Bauhinia brachycarpa and their inhibitory activities against α-glucosidase and pancreatic lipase.

Fitoterapia·2026

Same author

Multi-strategy feature selection and multi-model machine learning for prognostic prediction in primary gastric diffuse large B-cell lymphoma.

Digital health·2026

Same author

Virtual energy shortage risk to trade network in China.

Fundamental research·2026

Same author

Data-driven and physics-informed estimation of magnetic nanoparticle properties via stochastic Langevin model.

Nanotechnology·2026

Same author

Magnetic nanoparticles for cancer theranostics.

Biomedical physics & engineering express·2026

Same author

Integrated bioactivity-guided isolation, network pharmacology, and experimental validation to reveal the key constituents and potential mechanisms of Tectona grandis against diabetes and lipid disorders.

Journal of ethnopharmacology·2026

Search research articles

Related Experiment Video

Updated: Feb 6, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

A novel polysaccharide from Wisteriopsis eurybotrya: extraction optimization, structural characterization, and

Yuping Chen¹, Hanlei Wang¹, Xia Zhao²

¹Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, 650223, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Carbohydrate Research

|February 4, 2026

Summary

Wisteriopsis eurybotrya polysaccharides (WEP) were optimized for extraction. A purified fraction, WEP-1-1, showed significant hypoglycemic and immunomodulatory effects, making it a promising natural functional material.

Keywords:

Extraction optimization Hypoglycemic activity Immunomodulatory activity Polysaccharide Structure

More Related Videos

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

Published on: October 23, 2015

Related Experiment Videos

Last Updated: Feb 6, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Published on: March 17, 2023

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

Fabrication of a Bioactive, PCL-based "Self-fitting" Shape Memory Polymer Scaffold

Published on: October 23, 2015

Area of Science:

Plant-derived polysaccharides
Biochemistry
Immunology

Background:

Polysaccharides from Wisteriopsis eurybotrya (WEP) are of interest for their potential health benefits.
Optimizing extraction conditions is crucial for maximizing yield and activity.
Understanding the structure-activity relationship is key to developing functional materials.

Purpose of the Study:

To optimize hot-water extraction conditions for Wisteriopsis eurybotrya polysaccharides (WEP).
To isolate and characterize a purified polysaccharide fraction (WEP-1-1).
To evaluate the in vitro hypoglycemic and immunomodulatory activities of WEP-1-1.

Main Methods:

Single-factor tests and response surface methodology were used to optimize WEP extraction.
WEP-1-1 was purified and its structure was characterized using techniques like molecular weight determination and monosaccharide composition analysis.
In vitro assays were performed on C2C12 myotubes, L02 cells, and RAW264.7 cells to assess glucose uptake and immune responses (phagocytosis, NO, TNF-α production).

Main Results:

Optimal extraction conditions for WEP were determined as 92°C, 241 min, and a 43 mL/g liquid-solid ratio.
Purified WEP-1-1 had a weight-average molecular weight of 130.3 kDa and was composed of Glc, Ara, Gal, Rha, Xyl, and Man.
WEP-1-1 significantly enhanced glucose uptake in myotubes and liver cells, and boosted phagocytosis and pro-inflammatory cytokine production in macrophages.

Conclusions:

WEP-1-1 exhibits significant hypoglycemic activity by promoting glucose uptake.
WEP-1-1 demonstrates potent immunostimulatory effects, enhancing cellular immune responses.
WEP-1-1 is a promising natural functional material with dual hypoglycemic and immunomodulatory properties.