A nanosensor platform for cell gene transfection and single-cell nanovibration dynamic monitoring

Cell transfection and dynamic monitoring of subsequent cellular physiological activity play a crucial role in studying gene function and regulating gene expression. However, platforms integrating cell transfection with dynamic monitoring of cellular physiological activity are rarely reported. Here, we propose a novel technology using a nanopore array platform combined with nanodrums fabricated from ultrathin graphene, which enables single-cell transfection while dynamically monitoring post-transfection changes in cellular physiological activity. In our exploration, we found that random oscillation amplitudes generated by single OCI-Ly3 cells reached up to ∼20 nm. After transfection with the ME2 gene knockdown sequence (shME2), cellular physiological activity was inhibited, and the random oscillations were suppressed, with amplitudes decreasing to < 5 nm. Our results provide a high-throughput potential and efficient approach for gene transfection and dynamic monitoring of cellular physiological activity, offering valuable insights for cell-based drug screening, cancer cell subtype classification, and other applications.