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Updated: Feb 6, 2026

Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
Published on: November 8, 2024
Characterization, organoid formation and differentiation potential of the novel term trophoblast cell line
Gudrun Meinhardt1, Hanna Waldhäusl1, Leila Saleh1
1Department of Obstetrics and Gynaecology, Reproductive Biology Unit, Placental Development Group, Medical University of Vienna, Vienna, Austria.
Abstract:
Herein, we molecularly characterize the novel human term trophoblast cell line TB/SVTERT350 and show that it meets the criteria for trophoblast identity and subtype specification. TB/SVTERT350, cultured under stemness conditions in 2D or 3D, express trophoblast progenitor markers such as E-cadherin, TEAD4 and YAP1 and develop into CGβ-producing syncytiotrophoblast and HLA-G-expressing extravillous trophoblasts in the respective differentiation media. Expanding TB/SVTERT350 organoids share structural similarities with primary trophoblast organoids and express NOTCH1 suggesting that the cell line exhibits phenotypic traits of proximal cell column trophoblasts. In summary, TB/SVTERT350 could represent a reliable model for studying mechanisms of placental growth and differentiation.
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