MgO nanoparticles formulated from konjac glucomannan and its therapeutic potential

Konjac glucomannan (KGM) was utilized as a novel, natural capping and stabilizing agent for the green synthesis of magnesium oxide nanoparticles (MgO NPs) with potential biomedical applications. The synthesized KGM-MgO NPs were predominantly spherical with mild aggregation and an average particle size of 13.34 nm, as confirmed by transmission electron microscopy. The biological performance of the nanoparticles was evaluated through antioxidant, hemocompatibility, and cytotoxicity assays, together with in vivo wound healing, in ovo angiogenesis, and phytotoxicity studies. The KGM-MgO NPs exhibited moderate antioxidant activity and remained non-hemolytic up to 100 μg/mL, while inducing a concentration-dependent reduction in viability of A2780 ovarian cancer cells. In vivo wound healing experiments demonstrated that a 1 wt% KGM-MgO NPs formulation significantly enhanced wound contraction (82% by day 14), accompanied by improved collagen deposition and re-epithelialization. No adverse effects on vascular architecture were observed in the chick chorioallantoic membrane model, and no phytotoxic effects were detected in Vigna radiata. These findings establish KGM as an effective biopolymer capping agent for MgO nanoparticles and support their suitability for wound healing and related biomedical applications.