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Updated: Feb 6, 2026

Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
Published on: June 27, 2018
A self-cleavable ULP1-SUMO fusion strategy for enhanced soluble protein expression and one-step purification in
Changbin Jin1, Xiaohu Zhang1, Zhibo Yang1
1State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Abstract:
The Escherichia coli (E. coli) expression system is widely used for recombinant protein production; however, protein insolubility remains a major challenge. Although fusion tags can enhance solubility, they often complicate purification due to the need for tag removal. Here, we demonstrate that tandem fusion of Trigger Factor (TF) and SUMO markedly improves the solubility and expression of feline junctional adhesion molecule A (fJAMA) and Leptotrichia wadei CRISPR-associated protein 13a (LwCas13a). Furthermore, we developed a novel self-cleaving tandem tag by fusing SUMO protease Ulp1 to SUMO, enabling autonomous tag removal during expression and purification. This integrated design allows one-step purification of target proteins, thereby streamlining the workflow and reducing both time and cost.
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