Related Experiment Video
Updated: Feb 6, 2026

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
Published on: February 13, 2013
Thrombin generation and thrombin dynamics are associated with recurrent venous thromboembolism after anticoagulation
Geke C Poolen1, Romy M W de Laat-Kremers2, Mark Roest3
1Center for Benign Haematology, Thrombosis and Haemostasis, Van Creveldkliniek, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.
Background:
Recurrent venous thromboembolism (VTE) is a major concern after stopping anticoagulation. Thrombin generation (TG) parameters-including thrombomodulin (TM) sensitivity-have been proposed as biomarkers of recurrence, but findings remain inconsistent. The role of thrombin dynamic (TD) parameters, including prothrombin conversion and thrombin inactivation, is unclear.
Objectives:
This study evaluated whether TG and TD parameters are associated with VTE recurrence.
Methods:
In 589 patients from the prospective VISTA study, TG was measured during anticoagulation and 1 month after stopping vitamin K antagonist therapy. TM sensitivity was quantified as the percentage inhibition of endogenous thrombin potential (ETP) and peak thrombin concentration. TD parameters were derived computationally from TG curves. Cox regression was used to evaluate associations with recurrent VTE >2 years, including subgroup analyses excluding hormone-related events.
Results:
During the 2-year follow-up, 63 patients experienced recurrent VTE. In multivariable Cox regression adjusted for age, sex, index event type, and hormone use, reduced thrombin inactivation by antithrombin was associated with recurrence (per 100-unit decrease-hazards ratio [HR], 1.10; 95% CI, 1.00-1.20). In patients without hormone-related VTE, lower ETP and reduced TM-mediated inhibition of peak thrombin concentration were also significantly associated with recurrence (ETP per 100-unit decrease-HR, 1.11; 95% CI, 1.01-1.21; TM sensitivity per 10% decrease-HR, 1.26; 95% CI, 1.00-1.58). Other TG parameters were not significantly associated with recurrence.
Conclusion:
Reduced ETP, TM sensitivity, and thrombin inactivation were associated with VTE recurrence in people with unprovoked VTE. These biomarkers may have potential to identify patients at higher risk of recurrence.
More Related Videos
06:35Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research
Published on: March 29, 2024
07:08Assessment of the Anticoagulant and Anti-inflammatory Properties of Endothelial Cells Using 3D Cell Culture and Non-anticoagulated Whole Blood
Published on: September 5, 2017
Related Concept Videos
Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...
Venous Return
What is Venous Return?
Venous return refers to the rate at which blood flows back to the heart from the body's peripheral veins. It's an integral part of the circulatory system...
Anticoagulant Drugs: Low-Molecular-Weight Heparins
Venous Thrombosis I: Introduction
Dynamic Equilibrium
Venous Thrombosis III: Interprofessional Care