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Updated: Feb 6, 2026

Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
Published on: February 17, 2023
Engineering scalable vascularized kidney organoids for in vivo glomerular filtration with human endothelial
Murat Tekguc1,2, Takuya Matsumoto1,2,3,4, Lukas M Altenburger2,5,6
1Nephrology Division, Massachusetts General Hospital, Boston, MA USA.
Abstract:
Kidney organoids derived from human pluripotent stem cells have emerged as promising models for studying kidney disease and therapeutic development. However, the lack of a scalable production system has limited their industrial applications in regenerative medicine. Here, we have developed a cost-effective mass-production method for manufacturing vascularized kidney organoids, which has improved production efficiency by more than 50 times compared to conventional culture systems. The incorporation of a dynamic culture environment in delta-wing stirred bioreactors has significantly enhanced the glomerular vascularization of kidney organoids via mechanosensory integrin α2β1. Single-cell RNA sequencing and functional analyses demonstrated the enhanced maturation in STR nephron epithelia. The large quantities of vascularized kidney organoids enabled the fabrication of a nephron sheet with nephron numbers equivalent to those found in two rat kidneys. Intravital imaging of a nephron sheet implanted in a dorsal skinfold chamber of mice revealed filtration function with size selectability in the organoid glomeruli vascularized with human endothelia. This work may represent a significant step towards bridging the gap between basic research and commercial products, paving the way towards developing bioengineered kidneys for kidney replacement therapy.
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