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Related Experiment Video

Updated: Feb 6, 2026

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
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Quantifying photothrombotic ischemic volume in the intact brain using light sheet microscopy.

Jessika Royea1,2,3, Catherine Albert1,2, Valerie Daignault1,2

  • 1Université de Montréal, École d'Optométrie, Montréal, Québec, Canada.

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|February 5, 2026
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Summary

Light sheet fluorescence microscopy (LSFM) offers a new, semi-automated method for precise stroke volume quantification in mouse brains. This technique accurately measures infarcts using autofluorescence, correlating well with existing methods.

Keywords:
ischemic volumelight sheet fluorescent microscopyphotothrombosisstroke

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Area of Science:

  • Neuroscience
  • Biomedical Imaging
  • Pathology

Background:

  • Photothrombotic stroke assessment traditionally uses in vivo imaging or ex vivo histology.
  • Existing methods like MRI and histology have limitations in resolution, motion artifacts, or tissue preservation.

Purpose of the Study:

  • To introduce and validate a semi-automated method for quantifying stroke volume using light sheet fluorescence microscopy (LSFM).
  • To assess the reliability and advantages of LSFM for experimental stroke research.

Main Methods:

  • Utilized LSFM for high-resolution, artifact-free imaging of optically cleared whole mouse brains after photothrombotic stroke.
  • Developed a semi-automated approach to delineate infarcts using endogenous autofluorescence.
  • Compared LSFM results with in vivo laser speckle contrast imaging, in vivo MRI, and cresyl violet histology.

Main Results:

  • LSFM accurately quantifies stroke volume by delineating infarcts via autofluorescence.
  • LSFM measurements showed strong correlations with established in vivo and ex vivo stroke assessment techniques.
  • The ischemic core's autofluorescence is preserved across different tissue preparation methods.

Conclusions:

  • LSFM provides a reliable, versatile, and powerful alternative for accurate stroke volume quantification.
  • This method preserves organ integrity and offers cellular-level resolution, overcoming limitations of traditional techniques.
  • LSFM is suitable for both fresh and stored samples, enhancing its applicability in experimental stroke research.