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Visualization of Calcium Decoding via a FRET-Based Reporter for Calcium-Decoder Conformation
Anja Liese1, Sarah Lederer2, Bernadette Eichstädt2
1Department for Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany. anja.liese@ipb-halle.de.
Abstract:
Calcium sensors as tools to visualize calcium dynamics have significantly advanced our understanding of calcium signaling, but visualization of calcium decoding remains a major challenge. Towards this end, we developed the CPKaleon reporter for monitoring conformational changes of the calcium sensor-decoder family of calcium-dependent protein kinases (CPK). It enables the real-time visualization of CPK activation and inactivation in response to specific treatments. Here, detailed protocols for the in vitro characterization and in vivo imaging of CPKaleon are presented.
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