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Visualization of Calcium Decoding via a FRET-Based Reporter for Calcium-Decoder Conformation.

Anja Liese1, Sarah Lederer2, Bernadette Eichstädt2

  • 1Department for Biochemistry of Plant Interactions, Leibniz Institute of Plant Biochemistry, Halle (Saale), Germany. anja.liese@ipb-halle.de.

Methods in Molecular Biology (Clifton, N.J.)
|February 5, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed CPKaleon, a novel reporter for visualizing calcium-dependent protein kinase (CPK) activation. This tool allows real-time monitoring of CPK conformational changes, advancing calcium signaling research.

Keywords:
BiosensorCPK conformational sensorCalcium-dependent protein kinases (CPKs)Förster resonance energy transferLive-cell imagingVisualization of calcium decoding

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Calcium signaling is crucial for cellular processes.
  • Visualizing calcium dynamics has advanced understanding, but calcium decoding remains challenging.
  • Calcium-dependent protein kinases (CPKs) play key roles in calcium signaling pathways.

Purpose of the Study:

  • To develop a reporter system for monitoring CPK activation and inactivation.
  • To enable real-time visualization of conformational changes in CPKs.
  • To provide detailed protocols for CPKaleon characterization and imaging.

Main Methods:

  • Development of the CPKaleon reporter system.
  • In vitro characterization of CPKaleon.
  • In vivo imaging of CPKaleon in cellular systems.

Main Results:

  • CPKaleon successfully monitors conformational changes of CPKs.
  • Real-time visualization of CPK activation and inactivation is achieved.
  • Detailed protocols for CPKaleon application are established.

Conclusions:

  • CPKaleon is a valuable tool for studying calcium decoding.
  • The reporter facilitates real-time monitoring of CPK activity in response to stimuli.
  • This work provides a foundation for further investigations into CPK-mediated signaling.