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Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models
Published on: October 22, 2012
Detection of Phosphorylated NADPH Oxidase by Antibody During Plant Immune Responses
Yuta Hino1, Hirofumi Yoshioka2
1Laboratory of Plant Immunology, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya, Aichi, Japan.
Abstract:
Reactive oxygen species (ROS) production during immune responses is tightly regulated by the multiple phosphorylation of plasma membrane-localized NADPH oxidases, respiratory burst oxidase homologs (RBOHs). It is known that calcium-dependent protein kinases (CDPKs) and other kinases phosphorylate N-terminal region of RBOH and activate ROS production during immune responses. In this chapter, we describe protocols for the preparation of anti-phosphopeptide antibodies, and the detection of phosphorylated RBOH during immune responses using anti-phospho-RBOH antibody in Nicotiana benthamiana.
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