Related Experiment Video

Updated: Feb 7, 2026

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants
05:54

Author Spotlight: Microscopic Analysis of Protein Localization at Plasmodesmata in Plants

Published on: November 1, 2024

2.6K

Blue Native-PAGE Assay of NLR Protein Complexes Combined with Genetic Complementation in Nicotiana benthamiana

Kodai Honda1, Tadashi Fujiwara1,2, Hiroaki Adachi3,4

  • 1Laboratory of Crop Evolution, Graduate School of Agriculture, Kyoto University, Muko, Kyoto, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|February 5, 2026
PubMed

Keywords:
AgroinfiltrationBN-PAGEComplementation assayNLRNicotiana benthamianaProtein complexWestern blotting

More Related Videos

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics07:55

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

14.8K
Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana13:29

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana

Published on: January 16, 2021

8.0K

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
Complement System01:27

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
10.8K
Complementation Tests00:49

Complementation Tests

A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
6.3K