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Updated: Feb 7, 2026
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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
Published on: January 3, 2025
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Genome Editing for Developing Disease-Resistant Plants
Muhammad Sohaib Shafique1, Yapei Liu1, Zhiyuan Ji2
1State Key Laboratory of Crop Gene Resources and Breeding/National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Methods in Molecular Biology (Clifton, N.J.)
|February 5, 2026
Summary
CRISPR-Cas9 genome editing enables precise modification of plant genomes to enhance disease resistance. This chapter details methods for developing disease-resistant crops using gene knockout and targeted gene insertion strategies.
Area of Science:
- Plant Science
- Genetics
- Biotechnology
Background:
- CRISPR-Cas9 technology revolutionizes crop breeding.
- Genome editing allows precise genetic modifications in plants.
- Enhancing disease resistance is crucial for crop improvement.
Purpose of the Study:
- To present CRISPR-based strategies for developing disease-resistant crops.
- To outline protocols for gene knockout and targeted gene insertion.
- To provide tools for accelerating the breeding of resistant cultivars.
Main Methods:
- Gene knockout of host susceptibility (S) genes.
- Targeted insertion of resistance alleles via homology-directed repair (HDR).
- Utilizing CRISPR-Cas9 for precise edits in crops like rice.
Main Results:
- Successful application of CRISPR-Cas9 for disease resistance engineering.
- Demonstrated efficacy of both gene knockout and HDR pathways.
- Development of comprehensive protocols for CRISPR-based crop improvement.
Conclusions:
- CRISPR-Cas9 offers a powerful platform for engineering plant immunity.
- These methods accelerate the development of disease-resistant crop varieties.
- Practical tools are provided for advanced plant breeding.
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