Related Experiment Video
Updated: Feb 7, 2026
10:02
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture 4C
Published on: April 29, 2020
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esBAF and INO80C fine-tune subcompartments and differentially regulate enhancer-promoter interactions
Braulio Bonilla1, Benjamin J Patty1, Snehal V Sambare2
1Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260 United States.
Genetics
|February 5, 2026
Summary
Two nucleosome remodelers, esBAF and INO80C, fine-tune 3D genome organization by altering subcompartment identity and enhancer-promoter communication at key regulatory loci in mouse stem cells.
Area of Science:
- Genomics
- Epigenetics
- Molecular Biology
Background:
- The genome's 3D structure is organized hierarchically, from chromosome territories to nucleosomes.
- Nucleosome remodelers mobilize histones, but their role in higher-order chromatin architecture is unclear.
Purpose of the Study:
- Investigate the roles of esBAF and INO80C nucleosome remodelers in 3D genome organization.
- Determine their impact on chromatin topology in mouse embryonic stem cells.
Main Methods:
- Utilized Hi-C and promoter capture Micro-C (PCMC) techniques.
- Analyzed changes in genome organization upon loss of esBAF or INO80C.
Main Results:
- Loss of esBAF or INO80C minimally affected global compartment and TAD structures.
- Subcompartment organization and promoter-anchored looping interactions were altered.
- Affected sites were enriched for bivalent chromatin regions bound by OSN, BRG1, and INO80.
Conclusions:
- esBAF and INO80C selectively influence subcompartment identity and enhancer-promoter communication.
- These remodelers play a crucial, previously underappreciated role in higher-order chromatin organization.
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