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Updated: Feb 7, 2026

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
Published on: April 30, 2019
Protocol for the quantitative imaging and segmentation of spatial patterns in Hibiscus trionum petals
Lucie Riglet1, Edwige Moyroud2
1The Sainsbury Laboratory, University of Cambridge, 47 Bateman Street, Cambridge CB2 1LR, UK.
None:
Most developmental processes underpinning the diversity of floral forms remain unexplored due to limitations in current imaging approaches. Here, we present a protocol to spatially analyze tissue-wide cell growth dynamics through confocal imaging and segmentation of the adaxial petal epidermis in Hibiscus trionum. We describe steps for dissecting buds, staining petals with a membrane dye, and acquiring images suitable for epidermal reconstruction. We detail procedures for image stitching and processing in ImageJ and for surface extraction and cell segmentation in MorphoGraphX. For complete details on the use and execution of this protocol, please refer to Riglet et al.1.
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