Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.9K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.9K
Viruses with RNA Genomes01:29

Viruses with RNA Genomes

935
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
935
Genomics02:02

Genomics

40.8K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.8K
Design Example: Strain Gauge Bridge or Wheatstone Bridge01:15

Design Example: Strain Gauge Bridge or Wheatstone Bridge

1.0K
The utilization of strain gauges as transducers for converting mechanical strain into electrical signals is a common practice in various engineering applications. These strain gauges are frequently integrated into Wheatstone bridge circuits to accurately measure parameters such as force or pressure. Within this context, each element within the circuit exhibits a resistance that undergoes subtle variations when subjected to mechanical strain. The primary objective is to convert minuscule...
1.0K
Wheatstone Bridge01:29

Wheatstone Bridge

1.2K
An ohmmeter is a resistance-measuring device. It works by applying a voltage to a resistor of unknown resistance and measuring the current across the resistor. The resistance value is deduced using Ohm's law. Usually, the standard configuration of an ohmmeter comprises a voltmeter or an ammeter. However, such configurations are limited in accuracy because the meters alter the voltage applied to the resistor and the current that flows through it.
Thus, for accurate resistance measurements, a...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural basis of RNA-guided DNA integration by type I CRISPR-associated transposases.

bioRxiv : the preprint server for biology·2026
Same author

Polyadenylation of insulin mRNA by Tent5a regulates pancreatic beta cells.

Nature communications·2026
Same author

Transposon end recognition and excision mechanisms of type I-F CRISPR-associated transposases.

bioRxiv : the preprint server for biology·2026
Same author

Prime editing of a pathogenic <i>Scn1a</i> allele ameliorates seizure phenotypes in a GEFS<sup>+</sup> mouse model.

Science translational medicine·2026
Same author

RNA-LNP-mediated in vivo prime editing corrects disease phenotypes in a mouse model of citrullinemia type I.

Science translational medicine·2026
Same author

Enhancing the performance of Magnets photosensors.

Nature communications·2026
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
See all related articles

Related Experiment Video

Updated: Feb 7, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.6K

Programmable genome editing in human cells using RNA-guided bridge recombinases.

Oana Pelea1, András Tálas2, Javier Fernández Carrera1

  • 1Department of Biochemistry, University of Zurich, Zurich, Switzerland.

Science (New York, N.Y.)
|February 5, 2026
PubMed
Summary
This summary is machine-generated.

A novel bridge recombinase, ISCro4, demonstrates high activity in human cells for precise gene editing. This technology enables efficient, site-specific DNA insertion and manipulation, advancing genome editing capabilities.

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.8K
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.2K

Related Experiment Videos

Last Updated: Feb 7, 2026

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.6K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.8K
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
09:51

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

14.2K

Area of Science:

  • Molecular Biology
  • Genome Engineering
  • Biotechnology

Background:

  • Site-specific insertion of large DNA fragments is a challenge in current genome editing.
  • IS110-family serine recombinases utilize bridge RNA for programmable DNA recombination.

Purpose of the Study:

  • To evaluate the activity and potential of the bridge recombinase ISCro4 in human cells.
  • To provide structural insights into ISCro4's enhanced activity.
  • To assess ISCro4's efficiency and specificity for genome editing applications.

Main Methods:

  • Expression and testing of ISCro4 in human cells.
  • Utilizing plasmid- and RNA-based delivery systems.
  • Assessing DNA excision, inversion, and insertion efficiencies.
  • Evaluating off-target activity and specificity.

Main Results:

  • ISCro4 shows high activity and enhanced function in human cells.
  • Programmable multi-kilobase DNA excisions and inversions were achieved.
  • Efficient donor DNA insertion (>6%) at targeted genomic sites was demonstrated.
  • Specificity and off-target analysis was performed.

Conclusions:

  • ISCro4 is a potent tool for advanced genome editing in human cells.
  • Bridge recombinases represent a promising next-generation genome editing technology.
  • Further development of bridge recombinases can expand editing capabilities beyond current limits.